Site-specific fluorescent labeling of proteins in vivo remains one of the most powerful techniques for imaging complex processes in live cells. Although fluorescent proteins in many colors are useful tools for tracking expression and localization of fusion proteins in cells, these relatively large tags (>220 aa) can perturb protein folding, trafficking and function. Much smaller genetically enc...

BACKGROUND Making peptide pharmaceuticals involves challenging processes where many barriers, which include production and manufacture, need to be overcome. A non common but interesting research area is related to peptides with intracellular targets, which opens up new possibilities, allowing the modulation of processes occurring within the cell or interference with signaling pathways. However,...

Recombinant clathrin protein fragments form assemblies that template gold nanocrystals in an array across the latticed surface. The nanocrystals exhibit unusual anisotropic morphologies with long range ordering, both of which are dependent upon the presence of a hexahistidine tag on the clathrin heavy chain fragments.

More than a century ago, Henry James authored The Golden Bowl, The Portrait of a Lady, and The Wings of the Dove—said to be the finest American prose fiction ever written (Bloom 2002:748). These novels are enthusiastically read and appreciated to this day, reflecting not only James’s innate artistry but also his development as a writer in response to critiques of his work. He once commented abo...

The function of pslD, which is part of the psl operon from Pseudomonas aeruginosa, was investigated in this study. The psl operon is involved in exopolysaccharide biosynthesis and biofilm formation. An isogenic marker-free pslD deletion mutant of P. aeruginosa PAO1 which was deficient in the formation of differentiated biofilms was generated. Expression of only the pslD gene coding region resto...

andre makine, a writer of russian ancestry residing in france, propounds the west enthusiastically and lyrically in his early novels. he visualizes the west as a lost paradise which is both fascinating and fanciful contrary to his childhood vision of russia. however, as time passes, following his immigration to france, his encounter with reality shatters and ruins the rudimental idealism which ...

In this paper, I use Witi Ihimaera’s reputation as a pioneer of Maori literature to analyze his negotiation of global and local influences on his writing in view of the claims of posterity and the obligation to the past. Ihimaera’s changing attitude is most discernible in his rewriting of his earliest novels, Tangi and Whanau, in The Rope of Man and Whanau II. Significant is the trope of the tr...

For a long time, writing novels is traditionally regarded as personal and private activities. People record their unique experience and emotions and project it into complicated characters and elaborated stories. However, there are other kinds of novels, called popular novels or internet novels, that are written to satisfy readers’ emotional need and demand. Despite various world settings, many ...