BACKGROUND Visualization of more than one antigen by multicolor immunostaining is often desirable or even necessary to explore spatial and temporal relationships of functional significance. Previously presented staining protocols have been limited to the visualization of three or four antigens. METHODS Immunofluorescence staining was performed both on slices of formalin-fixed tissue and on ce...

background: various prokaryotic and eukaryotic expression systems have been developed for the production of recombinant proteins. in the present study, we used a new protein expression system based on the iranian lizard leishmania, a trypanosomatid protozoan as a host, for the expression of lpg3 gene from leishmania infantum (l.infantum). methods: the lpg3 gene was cloned in the expression cass...

This study investigated the ability to reprogramming human fibroblast cells into hepatocyte-like by overexpressing HNF4a gene. The isolated and primary cultured from foreskin donated healthy patients. gene overexpressed through Doxycilin activatedthe vectors Tet-on-eGFP-HNF4a. after process were evaluated observing morphological changes, immunofluorescence staining assess expression of proteins...

This study was undertaken to investigate the distribution of mast cells (MCs) and the expression of transforming growth factor-β (TGF-β) on tryptase positive MCs in different types of human periapical diseases. Periapical tissues of 78 participates were used in this study, including healthy control (n=28), periapical cyst (n=25), and periapical granuloma (n=25). The tissue samples were fixed in...

BACKGROUND Obtaining high-quality images of cellular structures via immunofluorescence staining is critical for cellular localization studies. Often, these studies cannot be performed in parallel with certain oncology, virology, pharmacokinetic, and drug absorption studies due to model system technicalities requiring the cells to be cultured on porous membranes rather than glass or plastic. MAT...