Dopmine functionalized mesoporous onion-like silica (DPMS) was synthesized via a biomimetic coating, and lipase Candida sp. 99-125 (LCS) was immobilized in DPMS (LCS@DPMS) by physical adsorption in this study. The DPMS was characterized by SEM, TEM, BET and FT-IR, and it was shown that the DPMS had clear multishell structures with large surface area of 419 m2/g. The activity, pH stability, ther...

The scale-up of the ruthenium- and enzyme-catalyzed dynamic kinetic resolution (DKR) of (rac)-1-phenylethanol (2) is addressed. The immobilized lipase Candida antarctica lipase B (CALB) was employed for the resolution, which shows high enantioselectivity in the transesterification. The ruthenium catalyst used, (eta 5-C5Ph5)RuCl(CO)2 1, was shown to possess very high reactivity in the "in situ" ...

Bacillus subtilis lipase (BSL2) has been successfully immobilized into a Cu-BTC based hierarchically porous metal-organic framework material for the first time. The Cu-BTC hierarchically porous MOF material with large mesopore apertures is prepared conveniently by using a template-free strategy under mild conditions. The immobilized BSL2 presents high enzymatic activity and perfect reusability ...

Biodiesel is one alternative energy that might be promising to substitute the petroleum-based fuel due its biodegradability and less toxic level. Biodiesel can synthesized through non-alcohol route by substituting alcohol with methyl acetate using lipase enzyme as Biocatalyst, which may give by-product during reaction. In this research, from Bacillus subtilis ha...

Esterification and transesterification of high free fatty acid (FFA) oils to fatty acid alkyl ester (FAAE) by sol-gel entrapped lipase B from Candida antarctica (CalB) and lipase from Burkholderia cepacia were investigated. The optimal conditions for the esterification catalyzed by the immobilized CalB were determined to be 50 °C, 1.7:1 molar ratio of methanol to fatty acid, and 0.36 g of water...

Lipase from Candida rugosa (CRL) was immobilized by covalent attachment on hydrous niobium oxide. The matrix could effectively be attached to the enzyme with high retention of activity and prevent its leakage. Following immobilization, CRL exhibited improved storage stability and performed better at higher incubation temperatures. In addition, the enzyme retained most of its catalytic efficienc...

In this work, poly(n-butylamino)(allylamino)phosphazene (PBAP) was synthesized and tethered on polypropylene microporous membrane (PPMM) with the aim of offering a biocompatible and, at the same time, moderately hydrophobic microenvironment to lipase for the first time. Lipase from Candida rugosa was used and the influence of membrane surface conditions on the activities of immobilized lipases ...

Yarrowia lipolytica (YL) is a "non-conventional" yeast that is capable of producing important metabolites. One of the most important products that is secreted by this microorganism is lipase, a ubiquitous enzyme that has considerable industrial potential and can be used as a biocatalyst in the pharmaceutical, food, and environmental industries. In this work, Yarrowia lipolytica lipase (YLL) was...

Here, core-shell structured polydopamine-coated Fe3O4 nanoparticles were constructed to immobilize thermostable lipase QLM from Alcaligenes sp. Systematical characterization indicated that lipase QLM was successfully immobilized on the surface of nanoparticles with an enzyme loading of 21.4 ± 1.47 mg/g immobilized enzyme. Then, the immobilized enzyme was demonstrated to possess favorable cataly...

A cold adapted lipolytic bacterium Curtobacterium sp. was isolated from the soil of Gangotri glaciers, Western Himalayas. The optimum temperature and pH for cold active lipase activity was 15 C and 8.0 respectively. The effect of different carbon sources, organic and inorganic nitrogen sources on production of cold active lipase were studied. Glucose served as a good source of carbon for the pr...