Epidemiological studies have reported a higher incidence of rare disorders involving imprinted genes among children conceived using assisted reproductive technology (ART), suggesting that ART procedures may be disruptive to imprinted gene methylation patterns. We examined intra- and inter-individual variation in DNA methylation at the differentially methylated regions (DMRs) of the IGF2/H19 and...

References: 1) Oshima,A. et al. (1988) J. Biol. Chem. 263, 2553-2562. 2) Laureys.G. etal. (1988) Genomicsi, 224-229. 2/162 6/162 129/162 20/162 1/162 4/162 0.01 0.04 0.80 0.12 0.01 0.02 Source/Description: The clone VAW140 (1) was tested for CA repeats by Southern blot hybridization (2). One CA-hybridizing Hindm fragment was subcloned into mp8 in both orientations and sequenced at each end. A (...

αU (R) ⊆ R] ⇐⇒ { (ρ, a(i)) | (ρ, a) ∈ R, i ∈ [0, ρ(N)− 1] } ⊆ R] ⇐⇒ ∀(ρ, a) ∈ R : ∀i ∈ [0, ρ(N)− 1] : (ρ, a(i)) ∈ R] ⇐⇒ (ρ, a) ∈ R =⇒ ∀i ∈ [0, ρ(N)− 1] : (ρ, a(i)) ∈ R] ⇐⇒ R ⊆ { (ρ, a) | ∀i ∈ [0, ρ(N)− 1] : (ρ, a(i)) ∈ R] } ⇐⇒ R ⊆ γU (R]) Actually, we have a Galois embedding as αU is onto: any (ρ, x) ∈ EU is the abstraction αU ({ (ρ, a) }) of some (ρ, a) ∈ E such that ∀i ∈ [0, ρ(N)− 1] : a(i) = x.

Methylation of CpGs is generally thought to repress transcription without significant influence from the sequence surrounding the methylated dinucleotides. Using the mouse Igf2/H19 imprinting control region (ICR), Igf2r differentially methylated region 2 (DMR2) and bacterial sequences, we addressed how methylation-dependent repression (MDR) from a distance varies with CpG number, density and su...

The kinship theory of genomic imprinting proposes that parent-specific gene expression evolves at a locus because a gene's level of expression in one individual has fitness effects on other individuals who have different probabilities of carrying the maternal and paternal alleles of the individual in which the gene is expressed. Therefore, natural selection favors different levels of expression...