background and objective: high-level gentamicin resistance (hlgr: mic ≥ 500 µg/ml) in enterococci is mediated by aminoglycoside modifying enzymes which is mainly encoded by aac(6′)-ie-aph(2′′)-ia gene. the aim of this study was to evaluate the frequency of aac(6′)-ie-aph(2′′)-ia gene in clinical isolates of enterococcus facium and enterococcus faecalis collected from hospitals in northwest of...