In this work, peptides were extracted from Silver carp (SC) scales via protease hydrolysis and separated using two membranes (M1 M2). The results revealed that the water: SC scale ratios of 50.6 mL/g, alkaline 1 (AP1) dose 2313.6 U/mL, pH 8.14 optimal conditions, peptide yield reached 88.77 ± 0.32%. conditions separation clarified: operating pressure M1 (M2) was 0.25 (0.4) MPa, liquid temperatu...

Cystatins are proteins, which inhibit cysteine proteases, such as papain. In this study, the 336-bp cystatin C gene (family II, HmCysC) of silver carp (Hypophthalmichthys molitrix) was cloned and expressed in Escherichia coli BL21 (DE3). HmCysC encodes mature peptide (HmCystatin C), with 111 amino acids. A typical QXXXG motif found HmCystatin it formed a cluster Cyprinus carpio Danio rerio phyl...

BACKGROUND Low-temperature techniques that prevent protein denaturation are being used to extract collagen from fish skin for cosmetic purposes. These extracts contain collagen with its triple helix structure preserved, as well as a number of other proteins. OBJECTIVES The aim of the study was to investigate collagen extracts from the skin of silver carp for the presence of small-molecule pep...

Silver carp (Hypophthalmichthys molitrix), grass carp (Ctenopharyngodon piceus), bighead carp (Aristichthys nobilis), and black carp (Mylopharyngodon piceus) rank first, second, fourth, and seventh in world fish production. In China, the Yangtze River harbours the most important natural populations of these species. We performed a polymerase chain reaction – restriction fragment length poymorph...