Genetic engineering methods have been used successfully to modify the fatty acid profile of elite Australian germplasm of Brassica napus and B. juncea. Co-suppression plasmids carrying oleate desaturase genes from each species have been constructed and transferred into Australian elite breeding lines of B. napus and B. juncea using Agrobacterium tumifaciens plant-transformation techniques. Modi...

The improvement of garlic plants (Allium sativum L.) via biotechnological approaches is currently limited by the lack of an applicable direct gene transfer system. In this paper, we present the development of a genetic transformation system using particle bombardment for gene delivery and immature clove-derived callus as the gene target. Plasmid DNA (pBI221.23), containing the selectable "hpt" ...

Gene silencing in plants inactivates trans-genes introduced into plants and/or endogenous homologous genes. This stable but potentially reversible loss of gene activity resembles epigenetic changes that occur in normal development. The stability of silencing implies the involvement of trans-acting components, although none of them have been identified so far. Here we report the finding of secon...

Rapidly growing embryogenic calli of different ages derived from the scutellum of mature seed embryos of indica rice (Rasi) and mature seeds of japonica rice (Taipei 309) was biolistically transformed. Plasmid pUCGUS containing the uidA gene encoding -glucuronidase was used to optimize transformation conditions using various combinations of helium pressure, target distance and gap distance. Pla...

The factors affecting the Agrobacterium mediated gene transformation of tomato cv. Riogrande are reported in detail. Shoot tips (explants) were transformed with pBI333 carrying rice chitinase (cht-3) gene. Approximately 49% transformation frequency was achieved. The optimum conditions for successful transformation were: infecting the explants with the Agrobacterium suspension for two minutes, c...

efficiency was not affected by DNA source or PCR condition. Blue colonies contained a blunt-end-ligated plasmid without insert. Twenty randomly chosen white colonies were sequenced. Nineteen of them contained inserts ended with a primer and an A/T pair formed by complementary overhangs, and one contained an abnormally formed blank plasmid that was missing a part of the original sequence. The nu...