In this paper, a road adaptive modified skyhook control for the semi-active Macpherson strut suspension system of hydraulic type is investigated. A new control-oriented model, which incorporates the rotational motion of the unsprung mass, is introduced. The control law extends the conventional skyhook-groundhook control scheme and schedules its gains for various road conditions. Using the verti...

We have expressed recombinant RNase P protein from Escherichia coli in high yield. A hexahistidine sequence at the amino terminus allowed protein purification in a single step. Mass spectrometry confirmed the molecular weight of the purified protein and indicated a purity of > 95%. Protein functionality was demonstrated by reconstitution of active holoenzyme.

Site-specific fluorescent labeling of proteins in vivo remains one of the most powerful techniques for imaging complex processes in live cells. Although fluorescent proteins in many colors are useful tools for tracking expression and localization of fusion proteins in cells, these relatively large tags (>220 aa) can perturb protein folding, trafficking and function. Much smaller genetically enc...

BACKGROUND Making peptide pharmaceuticals involves challenging processes where many barriers, which include production and manufacture, need to be overcome. A non common but interesting research area is related to peptides with intracellular targets, which opens up new possibilities, allowing the modulation of processes occurring within the cell or interference with signaling pathways. However,...

Recombinant clathrin protein fragments form assemblies that template gold nanocrystals in an array across the latticed surface. The nanocrystals exhibit unusual anisotropic morphologies with long range ordering, both of which are dependent upon the presence of a hexahistidine tag on the clathrin heavy chain fragments.

In this study, a cooperative control method based on model predictive and multiagent theory is proposed to an interconnected air suspension system with three controllable structures of interconnection mode, damping, vehicle height. The controller constructed discrete-time state-space model. optimal interval for force obtained through solving cost functions while satisfying set constraints contr...

This paper is concerned with full vehicle nonlinear active suspension systems, in which each suspension unit consists of three components: a nonlinear spring, a nonlinear damper and a nonlinear hydraulic actuator. An artificial intelligence Neural Control technique has been presented in this paper to design a robust controller for full vehicle nonlinear active suspension systems. The advantage ...

An electrical hydraulic control system (electro-hydraulic system) is thought to be a key component in excavator operation systems. Control methods with fixed parameters may not yield optimal performances because has various nonlinear uncertainties due the leakage and compressibility of fluid medium. Hence, novel PID controller based on improved differential evolution (IDE) introduced electro-hy...

Compared with the traditional automotive suspension, hydro-pneumatic suspension has characteristics of large energy storage ratio, nonlinear stiffness and can change ground clearance vehicle body, which makes have good ride comfort handling stability during driving. In order to improve performance it is necessary design test bench for test. Aiming at problem that output signal mechanical used i...

This paper chronicles the research on the design, analysis, experimentation and application of a high efficient, low-power variable stiffness suspension system. The central concept is based on a recently designed variable stiffness mechanism which consists of a horizontal control strut and a vertical strut. The horizontal strut is used to vary the load transfer ratio by controlling the location...