Immunoblot studies were performed on tissue extracts of rats injected with horseradish peroxidase (HRP) conjugates of wheat germ agglutinin (WGA) using anti-HRP and anti-WGA antisera. Immunoblots of uninjected, purified conjugates (WGA-HRP) demonstrated a 62-kilodalton (kd) conjugate (monomeric WGA conjugated to HRP). Other prominent immunobands included HRP (40 kd), monomeric WGA (22 kd), and ...

Methadone maintenance programs (MMP) for opioid dependence treatment have been widely used due to their effective therapeutic outcomes. Harm reduction programs (HRP) are complementary programs for severe patients with high risk behaviors and when abstinence is not possible. This study aims to compare patients in MMP that use HRP (MMP-HRP) and patients in MMP who do not use HRP (MMP-NO HRP). The...

Horseradish peroxidase (HRP) is used in various biotechnological and medical applications. Since its isolation from plant provides several disadvantages, the bacterium Escherichia coli was tested as recombinant expression host in former studies. However, neither production from refolded inclusion bodies nor active enzyme expression in the periplasm exceeded final titres of 10mg per litre cultiv...

the binding behavior of a competitive inhibitor, n-phenylbenzhydroxamic acid (bha) against horseradish peroxidase (hrp) was studied in order to understand and predict the interaction mechanism of hydrogen donors with the enzyme. the dissociation constants of the complexes of hrp-bha, hrp-donor and hrp-bha-azide were estimated at specified conditions by difference spectroscopy. the binding site ...

Handle region peptide (HRP), which was recognized as a blocker of (pro)renin receptor ((P)RR), may block the function of (P)RR. The aim of this study was to investigate the effect of HRP with a large dose of 1 mg/kg/d on glucose status in the rats treated neonatally with monosodium L-glutamate (MSG). At the age of 8 weeks, the MSG rats were randomly divided into MSG control group, HRP treated g...

As an emerging nonthermal technology, cold plasma has been used to inactivate endogenous enzymes that are responsible for enzymatic browning reaction of fruits and vegetables. This study aimed investigate the inactivation effect dielectric barrier discharge (DBD) on horseradish peroxidase (HRP), a typical plant peroxidase. The results showed DBD caused HRP in time-and power-dependent manner. ac...

This paper presents a prototype humanoid robotics platform developed for HRP-2. HRP-2 is a new humanoid robotics platform, which we have been developing in phase two of HRP. HRP is a humanoid robotics project, which has been lunched by Ministry of Economy, Trade and Industry (METI) of Japan from 1998FY to 2002FY for five years. The ability of the biped locomotion of HRP-2 is improved so that HR...

Hepatoma-derived growth factor-related protein-3 (Hdgfrp3 or HRP-3) was recently reported as a neurotrophic factor and is upregulated in hepatocellular carcinoma to promote cancer cell survival. Here we identified HRP-3 as a new endothelial ligand and characterized its in vitro and in vivo functional roles and molecular signaling. We combined open reading frame phage display with multi-round in...

The direct coupling of the carboxylic derivative of steroids to the amino group of enzymes is a well-established method in steroid enzyme immunoassays for making enzyme conjugates (1). Horseradish peroxidase (HRP), containing six lysine residues in the sequence, is a widely used enzyme in enzyme immunoassays; in practice, however , only one or two of these are generally available for reaction (...

Horseradish peroxidase (HRP) and thionine (Th) were co-adsorbed onto a porous carbon-felt (CF) from a mixed solution of HRP and Th to fabricate a CF-based bioelectrocatalytic flow-detector for H(2)O(2). The resulting HRP and Th co-adsorbed CF (HRP/Th-CF) was successfully used as a working electrode unit of a highly sensitive amperometric flow-H(2)O(2)-biosensor. Differing from the ordinary medi...