Amperometric hydrogen peroxide biosensors were fabricated by incorporating horseradish peroxidase (HRP) into poly[pyrrole-co-[4-(3-pyrrolyl)butanesulfonate]] (Py-PS) copolymer films deposited on an SnO2 electrode surface by electropolymerization. The HRP/Py-PS electrodes exhibited an extended dynamic range and a markedly improved operational and storage stability, compared with HRP-incorporated...

This paper presents an advanced leg module developed for HRP-2. HRP-2 is a new humanoid robotics platform, which we have been developing in phase two of HRP. HRP is a humanoid robotics project, which has been lunched by Ministry of Economy, Trade and Industry (METI) of Japan from 1998FY to 2002FY for five years. The ability of the biped locomotion of HRP-2 is improved so that HRP-2 can cope wit...

Horseradish and soybean peroxidases (HRP and SBP, respectively) are useful biotechnological tools. HRP is often termed the classical plant heme peroxidase and although it has been studied for decades, our understanding has deepened since its cloning and subsequent expression, enabling numerous mutational and protein engineering studies. SBP, however, has been neglected until recently, despite o...

Cholera toxin (CT), covalently attached to horseradish peroxidase (HRP), is a specific cytochemical marker for GM1 ganglioside (GM1) and retains the ability of the native toxin to raise levels of cyclic AMP in avian erythrocytes. Using a cytochemical stain for HRP, we found that 9% of control cultured murine neuroblastoma cells bound cholera toxin-horseradish peroxidase conjugates (CT-HRP) on t...

To investigate whether the putative (pro)renin receptor blocker, the handle region peptide (HRP), exerts effects on top of the blood pressure-lowering and cardioprotective effects of the renin inhibitor aliskiren, spontaneously hypertensive rats were implanted with telemetry transmitters to monitor heart rate and mean arterial pressure (MAP). After a 2-week recovery period, vehicle, aliskiren, ...

Hybrid DNA-protein nanogels represent potential protein vectors and enzymatic nanoreactors for modern biotechnology. Here, we describe a new, easy, robust method preparation of tunable with controllable size density. For this purpose, polymerase chain reaction is used to prepare highly biotinylated DNA as soft biopolymeric backbone, which can be efficiently cross-linked via streptavidin-biotin ...

Automatic home respiratory polygraphy (HRP) scoring functions can potentially confirm the diagnosis of sleep apnoea-hypopnoea syndrome (SAHS) (obviating technician scoring) in a substantial number of patients. The result would have important management and cost implications. The aim of this study was to determine the diagnostic cost-effectiveness of a sequential HRP scoring protocol (automatic ...

The in vitro interaction of soluble horseradish peroxidase (HRP) with homogeneous mono layers of mouse macrophages has been studied using sensitive biochemical and cytochemical techniques. The compartmentalization of HRP in extracellular and intracellular sites has been quantitatively evaluated. A significant fraction is bound to a serum-derived layer, which coats the surface of culture vessels...

Visualization of the neuronal tract tracer horseradish peroxidase (HRP) is commonly achieved through the histochemical detection of its enzymatic activity using 3,3',5,5'-tetramethylbenzidine (TMB) as a chromogen. However, the TMB product is unstable and is incompatible with tissue processing methods that render the enzyme inactive, or when a combination of HRP tract tracing with neuronal pheno...

this investigation was designed to determine the effect of intact ovalbumin and mannose-conjugatedovalbumin on the prevention of salmonella typhimurium adherence to the epithelium of small intestine ofchickens. mannose-conjugated ovalbumin was produced by maillard-type reaction between chickenovalbumin and d-mannose at 60°c. the results revealed that incubation up to 96 hrs caused the highestam...