in this study, a high-performance liquid chromatographic method (hplc) and uv spectrophotometric method were developed, validated and applied for the determination of theophylline in biological fluids. liquid- liquid extraction is performed for isolation of the drug and elimination of plasma and saliva interferences. urine samples were applied without any extraction. the chromatographic separat...

Validasi metode analisis adalah suatu proses dalam menjamin akurasi analisis, reprodusibel, spesifik dan stabil dari kisaran sesuai dengan tujuan penggunaan. mencakup semua prosedur yang menunjukkan pengukuran kuantitatif analit matriks biologis seperti plasma. Penentuan konsentrasi obat plasma merupakan hal kompleks karena kompleks. Metformin agen antihiperglikemik oral digunakan pengelolaan d...

This paper describes the development and evaluation of a HPLC and UV spectrophotometric methods to quantify Cefaclor Monohydrate in Oral suspensions and Capsules. HPLC analysis were carried out using a C18 Knauer column and a mobile phase composed of Triethylamine: methanol: Acetonitrile: water (2: 10: 20: 68)v\v%, with a flow rate of 1.0 mL/min and UV detection at 265 nm. For the spectrophotom...

Liquid-liquid extraction and solid phase followed by high-performance liquid chromatography (HPLC) connected with ultraviolet (UV) detection were used for the determination of phytohormones. The parameters influencing performance HPLC-UV method, including composition mobile gradient elution, column temperature, flow rate, wavelength, optimized. This method can simultaneously determine 11 phytoh...

Phenolic composition of green tea (Camellia sinensis) varies according to manufacturing processes that result in deglycosylation glycosylated phenolics and condensation, epimerization, degalloylation flavan-3-ols (catechins). Ambiguous phenolic assignments based on UV absorbance alone can occur when the chromatographic peaks overlapped slightly. We established an improved method using HPLC–UV c...

To validate the utility of on-line measurements of UV absorption for the direct quantitation of individual phospholipid molecular species isolated by reverse-phase HPLC, we synthesized 37 different individual molecular species of diacyl choline glycerophospholipids. UV absorbance response factors (integrate UV absorbance/nmole phospholipid) were calculated for all diacyl, alkenylacyl, and alkyl...