BACKGROUND This study was designed to obtain the chemical fingerprint and to investigate the effect of Phyllanthus urinaria on telomerase activity and apoptotic pathways in the human nasopharyngeal carcinoma cell line (NPC-BM1). MATERIALS AND METHODS The polyphenol compounds in P. urinaria were investigated by HPLC/MS. Cell viability with the treatment of P. urinaria, gallic acid, ellagic aci...

BACKGROUND/AIMS To study the spectrum-effect relationship and effective components of Ligusticum Chuanxiong Hort. (LCH) on the protection of canine myocardial ischemia. METHODS Fingerprint spectrum of LCH extracts was developed using high performance liquid chromatography (HPLC), and a canine model of acute myocardial ischemia was established by ligating the coronary artery. Bivariate correla...

A four-step development, optimization and validation strategy for high-performance liquid chromatography (HPLC) fingerprints of Ginkgo biloba extract is described. A suitable chromatographic system was selected first. The following step was performing a screening design to select important parameters. After selecting some controllable parameters and their range to further optimize, gradient opt...

In this paper we characterize the metabolic fingerprint and first reported anti-inflammatory activity of Hypericum gentianoides. H. gentianoides has a history of medical use by Native Americans, but it has been studied very little for biological activity. High-performance liquid chromatography (HPLC) and liquid chromatography-electrospray ionization-mass spectrometry (LC-ESI-MS) analyses of a m...

coronary artery disease (cad) is the most important cause of mortality and morbidity in the world. the knowledge of major risk factos can be useful in prevention of cad. there is no known major risk factof in many patients with myocardial infarction (mi). the dermatoglyphic pattern in patients with myocardial infarction is an interesting matter and little information is available about this rel...

In the present study, a rapid fingerprint analysis of Ligusticum chuanxiong (L. chuanxiong) was developed based on ultra-fast liquid chromatography coupled with diode array detection (UFLC-DAD). The analysis time was shortened from approximately 75 min on conventional high-performance liquid chromatography (HPLC) to 40 min on UFLC. This method was validated in terms of stability [<4.40% relativ...

Purpose: To study the influence of different sulfur fumigation time and dosage on the chemical constituents of Baizhi (Angelicae dahurica Radix). Methods: The relationship of chemical constituents in Baizhi with different sulfur fumigation time and dosages was evaluated by high performance liquid chromatography (HPLC) fingerprint and chemometrics methods, including similarity analysis (SA), hie...