A new method to produce hot-start conditions in PCR is described. Short double-stranded DNA fragments were found to inhibit the activity of DNA polymerases from Thermus aquaticus and Thermus flavus. This inhibition is not sequence specific, but exclusively dependent on the melting temperature of the fragments as shown by its correlation to their melting curves as measured. This property is expl...

Designing reliable CMOS chips involve careful circuit design with attention directed to some of the potential reliability problems such as electromigration and hot carrie:r effects. This paper considers logic synthesis to handle electromigration and hot carrier degradation early in the design. phase. The electromigration and the hot carier effects are est,imated at the gate level using signal a...

Quantum dots (QDs) are of great interest due to their unique chemical and physical properties. Recently, a hot start (HS) polymerase chain reaction (PCR) amplification performance based on QDs with a high-fidelity Pfu DNA polymerase has been reported. However, whether QDs can trigger HS effects with other high-fidelity or conventional DNA polymerases is yet to be understood. In the present stud...

New York University submitted two runs this year: a KBP Slot Filling run and a Cold Start run. The Slot Filling run used the same system as last year (Min et al. 2012), with only the minimal changes needed to address the revised specifications. These included changes in the provenance information and collapsing employee_of and member_of slots. We included the confidence estimation step (Li and ...

Radon therapy using radon (222Rn) gas is classified into two types of treatment: inhalation of radon gas and drinking water containing radon. Although short- or long-term intake of spa water is effective in increasing gastric mucosal blood flow, and spa water therapy is useful for treating chronic gastritis and gastric ulcer, the underlying mechanisms for and precise effects of radon protection...

A new technique of PCR hot start using oligonucleotide primers with a stem-loop structure is developed here. The molecular beacon oligonucleotide structure without any chromophore addition to the ends was used. The 3'-end sequence of the primers was complementary to the target and five or six nucleotides complementary to the 3'-end were added to the 5'-end. During preparation of the reaction mi...

Past research has shown robust relationships between neighborhood socioeconomic disadvantage and children's school achievement and social-emotional outcomes, yet the mechanisms for explaining these relationships are poorly understood. The present study uses data from 1,904 Head Start participants enrolled in the Head Start Impact Study to examine the role that classroom structural and relationa...

interventional techniques aim to find safer and better ways to treat vascular diseases even in many instances, the interventional radiology solutions has been considered the only treatment option for the patients. interventional radiologists are specialists who perform minimally invasive procedures instead of surgery or other treatments. these procedures apply various imaging and catheterizatio...