Heterologous radioimmunoassays for a semiquantitative analysis of al-microglobulin were developed, exploiting the binding between polyclonal rabbit or goat antisera against human, guinea pig, or rat almicroglobulin and ‘Z61-labeled human, guinea pig, or rat a,-microglobulin. Homologues of this protein were detected in human, guinea pig, Rhesus monkey, rat, mouse, rabbit, goat, horse, and cow se...

1. Complement is not fixed by immune aggregates resulting from the interaction of pneumococcus capsular polysaccharide and type-specific immune horse serum, although under proper conditions the substitution of immune rabbit serum gives positive results. 2. The negative results with immune horse serum are due to some poorly understood property of the specific antibodies rather than to some heter...

The effect of serum type and concentration on recombinant protein expression in vaccinia virus infected HeLa S3 cells was studied in both static and suspension culture. A model heterologous protein,β-galactosidase (β-gal), was used. Calf and horse sera in the range of 0.5-10%(v/v) were investigated. In static culture, the calf serum concentration did not show any significant influence on the β-...

mutannabi the well-known arab poet has dedicated many lines of his poetry to describing animals directly or indirectly. among the animals to whichmutanabbi has paid attention the horse has a very special status. most of the lines in which he has in some way described horses or horse riders are the lines about fights and battles in which the poet depicts the victories and bravery of the courageo...

Normal mammary epithelial cells from BALB/cfC3H midpregnant mice were freed from stromal cell types by Percoll density gradient centrifugation after collagenase digestion and were then embedded within collagen gels. Sustained growth leading to an increase in cell number was accomplished in response to cholera toxin and high concentrations of horse serum. The extent of growth was found to be dep...

In a previous paper (I) on the reaction of the guinea-pig to single and repeated doses of horse serum, I took the view that the violent reaction obtained at a second treatment which follows the first after a considerable incubation period, was due to the participation in the reaction of a specific reaction product or anti-body developed by the animal during the interval. In support of this posi...

Horse serum, crystallized hemoglobin, and crystallized egg albumin have been injected into the lung alveoli of dogs in which the entrances of the right lymphatics have been tied and the thoracic duct cannulated. Samples of blood and lymph have been taken following this injection. Only after several hours in the case of the horse serum and hemoglobin have these proteins been detected by immunolo...

One of the growth media in current use for Haemophilus ducreyi comprises Mueller Hinton agar, chocolatised horse blood, serum and IsoVitalex (BBL). For a better understanding of growth factors, attempts were made to simplify this complex medium. The horse blood was replaced by haemin (200 micrograms/ml), the serum by albumin (0.2%), and IsoVitalex was substituted only by L-glutamine 0.01%. Most...

Rabbits sensitized to horse serum developed a bacteremia of 9 to 12 hours' duration when they were inoculated simultaneously with normal horse serum and a strain of Streptococcus hemolyticus, while the bacteria could only be isolated from the blood stream of non-sensitized animals within the first 3 hours after inoculation. On the other hand, when antisera are employed as the antigen for shocki...

Purpose: The serum protein-binding characteristic of lamotrigine with sera from various species (horse, rat, rabbit and goat) was studied at different dielectric constants and temperatures to obtain an insight into the mechanism of interaction, evaluate the effect of dielectric constant on binding affinity, and to determine the effect of species variation on drug plasma-protein interaction. Met...