In this study, a series of 22 ring-substituted 1-hydroxynaphthalene-2-carboxanilides were prepared and characterized. Primary in vitro screening of the synthesized compounds was performed against Mycobacterium marinum, Mycobacterium kansasii and Mycobacterium smegmatis. The compounds were also tested for their activity related to inhibition of photosynthetic electron transport (PET) in spinach ...

The duration of the DNA synthesis stage (S phase) of the cell cycle is fundamental in our understanding of cell cycle kinetics, cell proliferation, and DNA replication timing programs. Most S-phase duration estimates that exist for plants are based on indirect measurements. We present a method for directly estimating S-phase duration by pulse-labeling root tips or actively dividing suspension c...

Mycobacterium marinum, a close relative of Mycobacterium tuberculosis is being used to study mycobacterial pathogenesis. M. marinum causes a systemic tuberculosis-like infection and disease in ectotherms such as frogs and fish. This review describes the development of M. marinum as a model pathogen and the more recent development of genetically tractable model hosts, namely the zebrafish, Droso...

Nicotianamine synthase (NAS), the key enzyme in the biosynthetic pathway for the mugineic acid family of phytosiderophores, catalyzes the trimerization of S-adenosylmethionine to form one molecule of nicotianamine. We purified NAS protein and isolated the genes nas1, nas2, nas3, nas4, nas5-1, nas5-2, and nas6, which encode NAS and NAS-like proteins from Fe-deficient barley (Hordeum vulgare L. c...

conclusions traditional preparation significantly decreased inflammation and accelerated wound healing in treated rats. furthermore, the findings of this study can be applied clinically in the future. background skin burn is one of the most common complications and remains a major public health issue worldwide. objectives this experiment was conducted to study the effects of traditional medicin...

A polar gibberellin active in the dwarf rice (Oryza sativa L.) seedling and barley (Hordeum vulgare L.) half-seed assays was extracted from immature sour cherry (Prunus cerasus L.) seed and identified as gibberellin A(32) by gas-liquid chromatography and selected ion monitoring.

The zebrafish, a genetically tractable model vertebrate, is naturally susceptible to tuberculosis caused by Mycobacterium marinum, a close genetic relative of the causative agent of human tuberculosis, Mycobacterium tuberculosis. We previously developed a zebrafish embryo-M. marinum infection model to study host-pathogen interactions in the context of innate immunity. Here, we have constructed ...

Both in vivo and in vitro, Mycobacterium marinum organisms were found to multiply within phagolysosomes of murine macrophages. It thus appears that M. marinum are neither killed nor inhibited from multiplying by lysosomal enzymes.