The current direct colorimetric assay for phytase activity in feeds has interference from high P background and other factors. Our objective was to develop a rapid and reliable spin column method to accurately determine phytase activity in feed ingredients or complete diets. After the feed sample was extracted by stirring in 0.2 M citrate buffer, pH 5.5, for 30 min at room temperature, the oily...

The majority of the P in cereal grains and oilseed meals is organically bound as phytic acid or phytate. This form of P is nutritionally unavailable to nonruminant animals due to the lack of phytase in their digestive tract. As a result, swine and poultry diets must be supplemented with highly available, inorganic sources of P to meet their P requirements. The poor bioavailability of P in the n...

Background. Phytate (myo-inositol hexakisphosphate) is a naturally occurring compound in plants. Due to chelation of several dietary minerals, it acts as a potent anti-nutrient in foods. Degradation of phytate has previously been found to significantly improve absorption of nutritionally important minerals, such as iron and zinc, in the human intestine. By removing regulatory genes for phytase ...

A feeding trial of ten weeks was conducted to evaluate the effect of microbial phytase supplementation on mineral digestibility in Labeo rohita fingerlings fed on canola meal-based diets. The experiment consisted of a reference diet and a basal diet. The reference diet was prepared according to the requirements of L. rohita and was used as a standard diet. The basal diet was made by replacing 3...

A feeding trial of ten weeks was conducted to evaluate the effect of microbial phytase supplementation on mineral digestibility in Labeo rohita fingerlings fed on canola meal-based diets. The experiment consisted of a reference diet and a basal diet. The reference diet was prepared according to the requirements of L. rohita and was used as a standard diet. The basal diet was made by replacing 3...

A 43-yr-old male presented with a 6-month history of episodes of coughing, shortness of breath and fever. He suffered from dyspnoea on minor exertion. The patient worked in a cattle feed factory and noticed that he had more complaints after his working hours. His symptoms could be ascribed to hypersensitivity pneumonitis due to contact with phytase, an enzyme added to cattle feed to strengthen ...

Soil samples of Bt Rhizosphere were collected from Bt cotton growing area of Andhra Pradesh, India and was used as a source material for isolation and screening of phytase producing bacteria. 19Bacteria were isolated from Bt Rhizosphere. Phytase enzyme activity of the cultures was screened on modified phytase solubulizing medium (MPSM). The result inferred that six isolates BtRS1 to BtRS6 were ...

Background: Addition of the phytase enzyme to the diet of monogastric animals is an effective and practical method for both utilization of phytate-P and P disposal. Besides hydrolytic effects on phytate, phytase may also improve the availability of minerals. Alkaline phosphatase (ALP) plays an important role in bone mineralization. Since dietary P levels decrease, serum ALP level has been shown...

Response surface methodology (RSM) was used to optimize the cultivation conditions for the production of phytase by recombinant Escherichia coli DH5α. The optimum predicted cultivation conditions for phytase production were at 3 hours seed age, a 2.5% inoculum level, an L-arabinose concentration of 0.20%, a cell concentration of 0.3 (as measured at 600 nm) and 17 hours post-induction time with ...

A study was conducted to investigate the effects of thermostable phytase supplementation on the growth performance and nutrient digestibility of broilers for 42 d. 1-day-old Arbor Acres chicks with similar initial body weight were randomly divided into 3 treatments consisting 6 pens of 20 chicks per pen. The control group was given maize-soybean meal basal diet and the other two groups were fed...