The most physiological type of cell cycle arrest - namely, contact inhibition in dense culture - is the least densely studied. Despite cell cycle arrest, confluent cells do not become senescent. We recently described that mTOR (target of rapamycin) is inactive in contact-inhibited cells. Therefore, conversion from reversible arrest to senescence (geroconversion) is suppressed. I this Perspectiv...

To gain a more complete understanding of hepatitis C virus (HCV) entry, we initially assessed the rate at which HCV initiates productive attachment/infection in vitro and discovered it to be slower than most viruses. Since HCV, including cell culture-derived HCV (HCVcc), exhibits a broad-density profile (1.01-1.16 g/ml), we hypothesized that the varying densities of the HCVcc particles present ...

Beta-carotene is a valuable carotenoid in high demand as a natural food coloring agent, provitamin A, additive to cosmetics, and health food. It can be accumulated up to more than 10% of cellular dry weight of Dunaliella salina under carotenogenic conditions such as high irradiance, high temperature, high salt concentration and nutrient deficiency. High beta-carotene productivity in Dunaliella ...

Plastic in any form is a nuisance to the well-being of the environment. The 'pestilence' caused by it is mainly due to its non-degradable nature. With the industrial boom and the population explosion, the usage of plastic products has increased. A steady increase has been observed in the use of plastic products, and this has accelerated the pollution. Several attempts have been made to curb the...

Objective Adult stem cells which are derived from different tissues, with their unique abilities to self-renew and differentiate into various phenotypes have the potential for cell therapy and tissue engineering. Human adipose tissue is an appropriate source of mesenchymal stem cells with wide differentiation potential for tissue engineering research. In this study isolated stem cells from hum...

BACKGROUND Bone engineering requires thicker three-dimensional constructs than the maximum thickness supported by standard cell-culture techniques (2 mm). A flow-perfusion bioreactor was developed to provide chemotransportation to thick (6 mm) scaffolds. METHODS Polyurethane scaffolds, seeded with murine preosteoblasts, were loaded into a novel bioreactor. Control scaffolds remained in static...

introduction: this study was initiated to examine the effect of the ampullary and the isthmic primary cell cultures of human oviduct during long-term co-culture on two-cell mouse embryos. materials and methods: using a mechanical procedure, epithelial cells from ampullary (a) and isthmic (i) regions of the human oviduct were isolated and cultured in ham's f-10 with 10% fetal calf serum (fsc). t...

background fish cell lines are advantageous alternatives to mammalian cell lines for carrying out in vitro research. they are used extensively for the study of fish biology, physiology and toxicology, and more recently for germplasm conservation of important fish species. objectives the present study aims to establish and characterize a novel fish cell line from muscle tissue of wallago attu ca...