Previous work on energy efficient broadcast protocols for wireless ad hoc networks are based a commonly used physical layer model called “Pathloss model” which assume two nodes can communicate if and only if they exist within their transmission radius. In this paper, we analyze the effect of realistic physical layer on energy efficient broadcast protocols. We employ a more realistic log-normal ...

Heptahelical receptors (HHRs) are generally thought to function as monomeric entities. Several HHRs such as somatostatin receptors (SSTRs), however, form homo- and heterooligomers when activated by ligand binding. By using dual fluorescent ligands simultaneously applied to live cells monotransfected with SSTR5 (R5) or SSTR1 (R1), or cotransfected with R5 and R1, we have analyzed the ligand rece...

The role of L- and D-isomers of homocysteine (Hcy) in vascular versus endocardial endothelial (EE) remodeling and function is not well understood. The hypothesis is that Hcy decreases EE cell density by activating matrix metalloproteinase (MMP) and by inducing left ventricular hypertrophy (LVH) in homocysteinemic hypertensive rats (HHR). And L- and D-isomers of Hcy have differential effects in ...

We report results from combined quantum mechanical/molecular mechanical (QM/MM) free energy simulations to explore metal-assisted phosphoryl transfer and general acid catalysis in the extended hammerhead ribozyme. The mechanisms considered here assume that the 2′OH group of C17 has already been activated (i.e., is deprotonated) and acts as a nucleophile to go on an inline attack to the adjacent...

The generation of Traditional Chinese Medicine (TCM) prescription is one the most challenging tasks in research intelligent TCM. Current researches usually use transfer learning methods to apply relevant technology text this task simply and roughly. Either they need train a model with large number standardized dataset, or ignore domain knowledge expertise In order solve these problems, we propo...

BACKGROUND Hormone receptors, including the estrogen receptor and progesterone receptor, human epidermal growth factor receptor 2 (HER2), and other biomarkers like Ki67, epidermal growth factor receptor (EGFR, also known as HER1), the androgen receptor, and p53, are key molecules in breast cancer. This study evaluated the relationship between HER2 and hormone receptors and explored the addition...

In this study, a tobramycin concentration-dependent whole-cell micro-biosensor (tob-HHAz) was constructed by fusing aptamer with hammerhead ribozyme (HHR) from Schistosoma mansoni. The biosensor obtained integrating all the modules into one complete RNA sequence, which easily introduced E. coli without suffering harsh external environments. Three independent tobramycin-sensitive structures were...

Small self-cleaving ribozymes are a group of natural RNAs that are capable of catalyzing their own and sequence-specific endonucleolytic cleavage. One of the most studied members is the hammerhead ribozyme (HHR), a catalytic RNA originally discovered in subviral plant pathogens but recently shown to reside in a myriad of genomes along the tree of life. In eukaryotes, most of the genomic HHRs se...

A combinatorial pharmacophore (CP) model for Multidrug and toxin extrusion 1 (MATE1/SLC47A1) inhibitors was developed based on a data set including 881 compounds. The CP model comprises four individual pharmacophore hypotheses, HHR1, DRR, HHR2 and AAAP, which can successfully identify the MATE1 inhibitors with an overall accuracy around 75%. The model emphasizes the importance of aromatic ring ...