Figure 1. A, Grocott methenamine silver (GMS) stain showing Pneumocystis organisms within a granuloma. Inset, digitally magnified Pneumocystis organism showing characteristic paired “dark bodies” diagnostic of Pneumocystis species. B, Immunohistochemical stain using antibodies directed against Pneumocystis species with use of the treptavidin-biotin immunoperoxidase method. The discreet nature o...

Histopathological study showed pseudoepitheliomatous hyperplasia of the epidermis and there was a granulomatous inlammatory iniltrate in the dermis composed of histiocytes, eosinophils, and polymorphonuclear cells. Globular structures with a thick, pigmented wall were seen scattered between the inlammatory cells, some of them arranged in the form of a rosary. hese structures stained with period...

Nocardia farcinica is a gram-positive, partially acid-fast, methenamine silver-positive aerobic actinomycete that is infrequently associated with nocardiosis. The relative frequency of Nocardia farcinica isolates in nocardiosis is unknown but thought to be under diagnosis. It is increasingly been recognized in immunocompetent patients. We report a case of disseminated Nocardia farcinica causing...

Purpose. Canaliculitis is classically associated with Actinomyces species, which are filamentous bacteria; the purpose of this study was to evaluate the extent to which nonfilamentous bacteria colonize canalicular concretions by using graded histopathological analysis. Methods. This is a series of 16 cases. The percentage of Gram-positive/Gomori's methenamine silver-positive filamentous bacteri...

A number of ultrastructural and cytochemical techniques were used to study intact epithelial cells lining the frog urinary bladder: high resolution autoradiography after administration of [3H]glucosamine or [3H]fucose; 125I iodination of external protein; concanavalin A-peroxidase, periodic acid-chromic acid silver methenamine; and colloidal thorium. Results indicate that the material (probably...