The successful encapsulation of human hepatocellular carcinoma (HepG2) cells would assist applications in tissue engineering. In this paper, we have developed a method to produce chitosan-alginate microfibers within a microfluidic chip. Also, by suspending cells in the mixing solution, we fabricated HepG2 cell-laden fibers. The encapsulated HepG2 cells in the chitosan–alginate fibers were more ...

Although various in vitro assays have been developed to evaluate the cytochrome P450 (CYP)-inducing potential of drug candidates, there is a continuing need for the development of a reliable model in drug discovery. The objective of the present study was to compare CYP induction by chemicals in HepG2 cells with Huh7, NKNT-3, and reverted NKNT-3 cells. HepG2 cells showed more similarity to human...

SREBP1c (sterol regulatory element-binding protein 1c) is a metabolic-syndrome-associated transcription factor that controls fatty acid biosynthesis under glucose/insulin stimulation. Oxidative stress increases lipid accumulation, which promotes the generation of reactive oxygen species (ROS). However, we know little about the role of oxidative stress in fatty acid biosynthesis. To clarify the ...

BACKGROUND The mechanism of rapid growth of the residual tumor after radiofrequency (RF) ablation is poorly understood. In this study, we investigated the effect of hyperthermia on HepG2 cells and generated a subline with enhanced viability and dys-regulated angiogenesis in vivo, which was used as a model to further determine the molecular mechanism of the rapid growth of residual HCC after RF ...

Carbon quantum dots assembled with gambogic acid via a π–π stacking interaction for cancer therapy have high drug loading and targeted delivery, which can overcome resistance, short circulation, retention in cells, thereby exhibiting significant inhibitory effect on the growth of HepG2 HeLa cells. This is reported by Jianfeng Wang co-workers article number 2200201.

The present study was carried out to evaluate the role of apoptotic proteins in REC-2006-mediated radiation protection in hepatoma cell lines. REC-2006 treatment 2 h before irradiation strongly inhibited the cleavage of ATM and PARP-1 in HepG2 cells. The expression of nuclear apoptosis inducing factor (AIF) was found to be more inhibited (~17%) in HepG2 cells in REC-2006 + radiation-treated gro...

background human telomerase reverse transcriptase (htert) has become an ideal target for development of anticancer therapy. small interfering rnas (sirnas) are very powerful reagents for gene silencing and show promise for cancer gene therapy. however, only a small number of sirnas have been demonstrated to be effective. for gene therapy targeting htert, it is essential to develop a robust syst...

microRNA-422a (miR-422a) is downregulated in many hematopoietic tumors and solid including hepatocellular carcinoma. We previously demonstrated that hepatitis B virus X protein (HBx) expression of miR-422a HCC cell line HepG2 vitro. However, we explore the mechanisms underlying this action. Forkhead box proteins (FOX) G1/Q1/E1 are known to negatively regulate expression, prompted us determine w...

The new anti-cancer and water-soluble curcumin derivatives have been synthesized solvent-freely by functionalizing the phenolic group of with various NH compounds to increase water solubility cancerous properties. biological applications drugs investigated against highly potent HepG2 carcinoma cell line. studies showed that could be used in low concentrations (µM/l<1) avoid anti-apoptoti...

Accumulating evidence indicates that Nanog plays a central role in modulating the biological behaviors of human hepatocellular carcinoma (HCC). However, the underlying mechanisms remain unclear. In the present study, we employed transcription activator-like effector nucleases (TALEN) to disrupt Nanog expression in HepG2 cells and obtained subcloned cells with diallelic Nanog mutations. Signific...