BACKGROUND AND OBJECTIVES The first hematopoietic stem cells (HSC) in the mouse able to give rise to the adult hematopoietic system emerge at embryonic day (E) 10.5 in the intraembryonic aorta-gonads-mesonephros (AGM) region, as demonstrated by transplantation into irradiated adult recipients. It has been shown by transplantation into conditioned neonatal or hematopoietic mutant adult recipient...

Polymerase micro (Polmicro) is an error-prone, DNA-directed DNA polymerase that participates in non-homologous end-joining (NHEJ) repair. In vivo, Polmicro deficiency results in impaired Vkappa-Jkappa recombination and altered somatic hypermutation and centroblast development. In Polmicro(-/-) mice, hematopoietic development was defective in several peripheral and bone marrow (BM) cell populati...

Human pluripotent stem cells (hPSCs)/OP9 coculture system is a widely used hematopoietic differentiation approach. The limited understanding of this process leads to its low efficiency. Thus, we used single-cell qPCR to reveal the gene expression profiles of individual CD34+ cells from different stages of differentiation. According to the dynamic gene expression of hematopoietic transcription f...

Hematopoietic cells emerge from hemogenic endothelium in the developing embryo. Mechanisms behind human hematopoietic stem and progenitor cell development remain unclear. Using a human pluripotent stem cell differentiation model, we report that cyclic AMP (cAMP) induction dramatically increases HSC-like cell frequencies. We show that hematopoietic cell generation requires cAMP signaling through...

Throughout development, hematopoietic stem cells migrate to specific microenvironments, where their fate is, in part, extrinsically controlled. CD44 standard as a member of the cell adhesion molecule family is extensively expressed within adult bone marrow and has been previously reported to play important roles in adult hematopoietic regulation via CD44 standard-ligand interactions. In this ma...

Потенциальная роль вирусов человека и бактериофагов после трансплантации гемопоэтических стволовых клеток: мини-обзорPossible role of intestinal human viruses and bacteriophages following hematopoietic stem cell transplantation: a mini-review

Changes in fecal microbiota affect the incidence and extent of graft-versus-host disease (GVHD) after allogeneic hematopoietic stem cell transplantation (HSCT). Most patients with hematological mal...

currently, hematopoietic stem cell transplantation (hsct) is the only curative option for patients with beta-thalassemia major, but liver iron overload in these patients will not decrease and hepatic fibrosis may still progress despite successful hsct. liver biopsy samples were taken from 14 patients (out of 25 patients) who underwent hsct. all patients met three criteria: negative hcv antibody...

conclusions chronic meningitis in immunocompromised hosts can be due to more than one pathologic etiology and we should do all diagnostic evaluation to rule out all possible differential diagnosis. introduction cryptococcal meningitis is a rare disorder that is caused by cryptococcus neoformans in immunocompromised host. case presentation the case was a 64-year-old man with a history of stem ce...

Rodriguez et al. present in this issue a cost-effectiveness analysis of Defibrotide (Defitelioi) for the treatment severe veno-occlusive disease context hematopoietic stem cell transpl...