Objective(s): Valproic acid (VPA) has proven to be as one of the most promising useful drug with anticancer properties.In this study, we investigate the VPA effects on E-cadherin expression in HeLa, TC1, MKN45, and HCT116 cell lines.  This study assesses the effects of VPA on human papillomavirus E7 expression in HPV positive cell lines. Materials and Methods: Cell lines were treated by2 mmol/...

objective: cancer is a major health problem worldwide and current therapies for cancer are often limited by short-term efficacy due to drug resistance. there has been much interest in the use of naturally occurring compounds with chemo-preventive and chemotherapeutic properties in the treatment of cancers. rose is one of the most important groups of ornamental plants which their fruits and flow...

background: while articular chondrocytes are among those appropriate candidates for cartilage regeneration, the cell dedifferentiation during monolayer culture has limited their application. several investigations have indicated the usefulness of alginate, but the topic of proliferation and differentiation of chondrocytes in alginate culture has still remained controversial. methods: rat articu...

1. Ornithine decarboxylase activity is stimulated in high-density HeLa-cell cultures by dilution of or replacement of spent culture medium with fresh medium containing 10% (v/v) horse serum. 2. After stimulation, ornithine decarboxylase activity reaches a peak at 4-6h, then rapidly declines to the low enzyme activity characteristic of quiescent cultures, where it remains during the remainder of...

background :wide distribution and low half-life of acyclovir has led to a high dose consumption of the drug. recent studies have shown that encapsulation of acyclovir in nano-carriers can increase effectiveness and decrease its side effects. we investigated the inhibitory effect of acyclovir loaded nano-niosomes against herpes simplex virus type-1 (hsv-1) in cell culture.   methods : in-vitro c...

  Background :Wide distribution and low half-life of acyclovir has led to a high dose consumption of the drug. Recent studies have shown that encapsulation of acyclovir in nano-carriers can increase effectiveness and decrease its side effects. We investigated the inhibitory effect of acyclovir loaded nano-niosomes against herpes simplex virus type-1 (HSV-1) in cell culture.   Methods : In-vitro...

We present a flexible and highly reproducible method using three-dimensional (3D) multicellular tumor spheroids to quantify chemotherapeutic and nanoparticle penetration properties in vitro. We generated HeLa cell-derived spheroids using the liquid overlay method. To properly characterize HeLa spheroids, scanning electron microscopy, transmission electron microscopy, and multiphoton microscopy ...

In an earlier study (Shepard, 1955) it was found that tubercle bacilli were much more actively phagocytosed by HeLa cells when horse serum was incorporated into the tissue culture medium, in place of the more commonly used human or rabbit serum. By selection of a favorable horse serum it could be seen that a significant proportion of the tubercle bacilli in an inoculum could thus be introduced ...

Zajac, Ihor (Hahnemann Medical College, Philadelphia, Pa.), and Richard L. Crowell. Location and regeneration of enterovirus receptors of HeLa cells. J. Bacteriol. 89:1097-1100. 1965.-Treatment of live HeLa cells with chymotrypsin or trypsin completely inactivated the viral receptors for coxsackievirus B3 and poliovirus T1, respectively. Enzyme-treated cells regained their enterovirus receptor ...

Tissue culture strains of cells from four different normal human tissues-liver, conjunctiva, kidney, and appendix-have been grown by the plating procedure previously developed for the HeLa strain of cervical carcinoma cells. This technique results in colony formation from isolated single cells, in a manner completely analogous to the plating of bacteria in semisolid nutrient media. Clonal cell ...