Disturbances in histone acetyltransferases (HATs) are common in cancers. In urothelial carcinoma (UC), p300 and CBP are often mutated, whereas the GNAT family HATs GCN5 and PCAF (General Control Nonderepressible 5, p300/CBP-Associated Factor) are often upregulated. Here, we explored the effects of specific siRNA-mediated knockdown of GCN5, PCAF or both in four UC cell lines (UCCs). Expression o...

With the rise of Web-based applications, it is both important and feasible for human-computer interaction practitioners to measure a product’s user experience. While quantifying user attitudes at a small scale has been heavily studied, in this industry case study, we detail best Happiness Tracking Surveys (HaTS) for collecting attitudinal data at a large scale directly in the product and over t...

OBJECTIVE To describe the development and activity of a multidisciplinary service to manage self-poisoning. DESIGN Descriptive, comparative study with prospective data collection. SETTING Regional toxicology treatment centre in the Hunter area of New South Wales (NSW) with primary and secondary referral service to 385,000 people and tertiary referral service to a further 100,000. PATIENTS...

Sequence similarity and profile searching tools were used to analyze the genome sequences of Arabidopsis thaliana, Saccharomyces cerevisiae, Schizosaccharomyces pombe, Caenorhabditis elegans and Drosophila melanogaster for genes encoding three families of histone deacetylase (HDAC) proteins and three families of histone acetyltransferase (HAT) proteins. Plants, animals and fungi were found to h...

Dynamic epigenetic regulation of neurons is emerging as a fundamental mechanism by which neurons adapt their transcriptional responses to specific developmental and environmental cues. While defects within the neural epigenome have traditionally been studied in the context of early developmental and heritable cognitive disorders, recent studies point to aberrant histone acetylation status as a ...

The development of B and T cells from hematopoietic precursors and the regulation of the functions of these immune cells are complex processes that involve highly regulated signaling pathways and transcriptional control. The signaling pathways and gene expression patterns that give rise to these developmental processes are coordinated, in part, by two opposing classes of broad-based enzymatic r...

Histone acetyltransferases (HATs) are a group of enzymes that acetylate lysine residues in histones, which are bound to DNA in all eukaryotes to form a complex and well organized structure called chromatin. HATs play an important role in regulating the chromatin organization and accessibility of the DNA to the transcriptional machinery by altering the acetylation status of histones in particula...