Trichoderma spp. cause large crop losses of the cultivated shiitake mushroom, Lentinula edodes. We bred several shiitake strains that are resistant to Trichoderma spp. using di-mon mating to establish a useful method for controlling the greenmold disease. We examined the competitive ability of L. edodes against Trichoderma spp. using a dual culture system to select resistant strains. By screeni...

A simple detached leaf-droplet inoculation bioassay was devised in order to investigate the potential for biological control of Botrytis cinerea by indigenous Trichoderma harzianum isolates. Following preliminary in vitro screening of the antagonistic capacity of four local isolates of Trichoderma harzianum (T1-T4) against B. cinerea, T3 spore suspensions were then tested for their ability to c...

Trichoderma species are widely used for the commercial production of cellulolytic enzymes. In the present investigation, medium components were optimized using a central composite design and response surface methodology to produce endoglucanase (EG) from Trichoderma harzianum KUC1716. From the various medium components tested, cellulose, soy peptone, and thiamine HCl were selected as the optima...

Soil and root samples of tomato were collected from root knot nematode-infested fields of fifteen different localities of Malakand and Swat (NWFP) Pakistan in 2006-07 and Trichoderma harzianum was isolated from these samples. These isolates were tested against Meloidogyne javanica (Treub) Chitwood in the in vitro. Isolates Th1 and Th9, collected from Jabban and Shamozai were more aggressive tha...

The present study was carried out to evaluate the ability of Trichoderma harzianum (ALL 42-isolated from Brazilian Cerrado soil) to promote common bean growth and to modulate its metabolism and defense response in the presence or absence of the phytopathogenic fungi Rhizoctonia solani and Fusarium solani using a proteomic approach. T. harzianum was able to promote common bean plants growth as s...

Twelve isolates of Trichoderma (six of T. harzianum, five of T. viride, one of T. virens), which reduced variably the incidence of collar rot disease caused in peanut by Aspergillus niger Van Tieghem, were evaluated for their potential to produce lytic enzymes during in vitro antagonism. T. viride 60 inhibited highest (86.2%) growth of test fungus followed by T. harzianum 2J (80.4%) at 6 days a...

Trichoderma harzianum strain ESALQ-1306 was assessed for its potential biological control against Meloidogyne incognita race 4 under in vitro and greenhouse conditions. In vitro, we noticed 64% of conidial attachment on eggs and second-stage juvenile immobilized by the fungus. In greenhouse, cucumber plants were treated with different concentrations of conidial suspensions before and after inoc...

Tolerance of Trichoderma species collected from tea plantation to fungicides was evaluated in vitro using poisoned food method. Fungicides like Carbendazim 50 WP, Copperoxychloride 50 WP, Hexaconazole 5 EC, and Propiconazole 25 EC were used at two lower and two upper of recommended doses. The growth of T. harzianum was mostly inhibited by the Carbendazim 50 WP and Propiconazole 25 EC with the r...

We have investigated the molecular basis for the reported synergism between peptaibols and cell wall hydrolytic enzymes in the antagonism of phytopathogenic fungi by Trichoderma harzianum. beta-Glucan synthase activity on isolated plasma membranes of Botrytis cinerea was inhibited in vitro by the peptaibols trichorzianin TA and TB, and this inhibition was reversed by the addition of phosphatidy...

Monoconidial cultures of 15 isolates of Trichoderma harzianum were characterized on the basis of 82 morphological, physiological, and biochemical features and 99 isoenzyme bands from seven enzyme systems. The results were subjected to numerical analysis which revealed four distinct groups. Representative sequences of the internal transcribed spacer 1 (ITS 1)-ITS 2 region in the ribosomal DNA ge...