DNA lesions, cytotoxicity, and cellular uptake of CaCrO4 were com pared in Chinese hamster ovary, in mouse embryo fibroblast C3H10T'/2, and in human osteosarcoma cells. The concentration of CaCrO4 that reduced colony formation by 50% was 2or 3-fold less in human osteo sarcoma cells than in C3H 10T'/2 or Chinese hamster ovary cells. Alkaline elution studies showed that CaCrO4 induced DNA single ...

background: irradiation is one of the major causes of induced sperm dna damage. various studies suggested a relation between sperm dna damage and fertilization rate after intra-cytoplasmic sperm injection (icsi). objective: in this study, fertilization rate and premature chromosome condensation (pcc) formation after icsi of hamster oocytes with irradiated sperms from normal and oligosperm indiv...

background: transient gene expression (tge) gained popularity over the last decade as a rapid method for the production of milligram to gram quantities of recombinant proteins for preclinical studies in biophama industry. thereby, the optimization of the tge technique for chinese hamster ovary (cho) as the dominant host for the production of biotherapeutics is of great interest to reach the val...

objective: development of high producing mammalian cell lines is a major bottleneck in manufacturing of recombinant therapeutic proteins. this study examines the effect of using the matrix attachment region from the human interferon beta gene in combination with promoter activation strategy with e1a 13s protein on human tissue plasminogen activator (t-pa) expression in chinese hamster ovary (ch...

MOST hepatic functions are genetically determined and. after liver transplantation. the hepatic parenchyma retains its species specificity. Therefore. in the hamster-to-rat liver xenotransplantation mode!.' we assessed the phenotypic changes of the serum protein profile in the recipient. as well as the functional compatibility between the xenoproteins and the recipient environment. Upon serum p...

DNA lesions, cytotoxicity, and cellular uptake of CaCrO4 were compared in Chinese hamster ovary, in mouse embryo fibroblast C3H10T1/2, and in human osteosarcoma cells. The concentration of CaCrO4 that reduced colony formation by 50% was 2- or 3-fold less in human osteosarcoma cells than in C3H10T1/2 or Chinese hamster ovary cells. Alkaline elution studies showed that CaCrO4 induced DNA single s...

than do human and rat liver, and hamster-derived cells in culture express more enzyme than do lines derived from tissues of other animals (18). This report describes the purification of 2 major glutathione S-transferase forms from hamster liver and a comparison of the properties of the forms with those from human and rat liver. The data suggest that certain fundamental differences between the h...

Hamster cells which transformed after exposure to herpes simplex virus type 2 previously irradiated with ultraviolet light were tested for the presence of hamster leukosis viruses or their virus-specific antigens. Three techniques that were used did not detect the presence of either hamster C-type particles or antigens commonly associated with such structures. The implication of these findings ...

Cytoplasmic retinoic acid binding protein (cRABP) is present in human fetal pancreas and becomes nondetectable in the normal adult pancreas. The binding protein for retinoic acid becomes apparent again in pancreatic cancer. Similar fluctuations in the content of cRABP occur in the hamster. The binding protein is undetectable in the normal adult hamster pancreas, while it was detected in several...