We evaluated extracts taken from S. magellanica third instar larvae fat body and haemolymph using a diabetic rabbit model and compared this to the effect obtained with the same substances taken from Lucilia sericata larvae. Alloxan (a toxic glucose analogue) was used to induce experimental diabetes in twelve rabbits. Dorsal wounds were made in each animal and they were infected with Staphylococ...

1. Models developed to predict behavioural and life-history decisions of parasitoids rely heavily on assumptions concerning the nature and function of the nutrients that the female obtains from the host. 2. Using a combination of colorimetric and thin-layer chromatography techniques, we analysed the composition of the host-feeding fluid consumed by the parasitoid Eupelmus vuilletti and found it...

Terrestrial arthropods, at constant risk from desiccation, are highly sensitive to atmospheric temperature and humidity. A physiological marker of these abiotic conditions could highlight phenotypic adaptations, indicate niche partitioning, and predict responses to climate change for a group representing three-quarters of the Earth's animal species. We show that the (18)O composition of insect ...

A recently isolated haemolymph protein appears to be an important regulator of ecdysone biosynthesis by prothoracic glands in Manduca sexta. Using a dose-response titration protocol, the haemolymph titre of this stimulatory factor was determined during the last larval instar. The titre was high (greater than 2.0 U ml-1) on days 0 and 1, then dropped significantly to 0.55 U ml-1 on day 2, and re...

Microanalytical techniques are used to show that organic molecules play an important role is osmoregulation. Changes in the inorganic ion composition of haemolymph from fourth instar larvae of Aedes aegypt} are correlated with the changes in the concentration of organic ions. Free amino acids have a significant role in regulating haemolymph osmotic pressure with respect to the osmotic pressure ...

The desert locust Schistocerca gregaria is a destructive pest for several crops, particularly which are considered as the main food sources human and animals. Much attention has been paid to use plant extracts or products controlling this pest. present study aimed investigate disturbing effect of Nimbecidine on phosphatase activity in S. gregaria. penultimate instar nymphs were treated, through...

1. The drinking rate of the saline-water mosquito larva Aedes taeniorhyncus (100 nl.mg-1.h-1) is unaffected by the salinity of the external medium, but is directly proportional to the surface area of the animal. 2. Haemolymph Na+, Mg2+, K+, Cl-, SO42- and osmotic concentrations were measured in larvae adapted to 10%, 100% and 200% seawater and were found to be regulated within a narrow range. ...

Immunoadsorption, Ecdysteroids, Calliphorin, Steroid-Binding Protein, Photoaffinity-Labelling Endogenous ecdysteroids as well as radiolabelled exogenous ecdysone were crosslinked to a protein when haemolymph from blowfly larvae was irradiated with UV-light under optimized conditions. This indicates that larval haemolymph of blowflies contains an ecdysteroid-binding protein. The crosslinked ecdy...

-1. Amounts of glycogen and glucose in locust haemolymph and fat body increase during the first 15 rain of flight but later fall to below the resting values. 2. Amounts of trehalose in haemolymph and glycogen in gut wall decline throughout flight. 3. Variations in the concentrations of free amino acids in haemolymph are reported. 4. The concentration of lipid in the haemolymph increases to abou...