Background and objective Acute ischemic stroke is a potentially devastating condition leading cause of morbidity mortality, affecting an estimated 800 000 people per year in the USA. The natural history untreated or unrevascularized large vessel occlusions acute patients results mortality rates approaching 30%, with only 25% achieving good neurologic outcomes at 90 days. Recently, data...

B7-H3 protein is an important tumor antigen, but the expression of its isoforms, 4IgB7-H3 and 2IgB7-H3, in tumor tissues remains unknown due to the lack of specific monoclonal antibodies (mAbs). In the present study, a mAb (9C3) specifically recognizing 2IgB7-H3, but not 4IgB7-H3, was prepared. Using 9C3 and a previously prepared mAb (4H7) that recognizes 4IgB7-H3 and 2IgB7-H3, the differential...

Post-translational modifications of individual lysine residues of core histones can exert unique functional consequences. For example, methylation of histone H3 at lysine 79 (H3-meK79) has been implicated recently in gene silencing in Saccharomyces cerevisiae. However, the distribution and function of H3-meK79 in mammalian chromatin are not known. We found that H3-meK79 has a variable distribut...

AbstractBackground The coronavirus disease 2019 (COVID-19) pandemic has resulted in the disruption of provision human immunodeficiency virus (HIV) services. This study examined factors associated with difficulties accessing HIV services during COVID-19 pandemic. Methods An online survey 236 Chinese-speaking gay and bisexual men Hong Kong conducted 2020. Results</h...

AbstractPurpose We aimed to systematically analyse how the radiology community discusses concept of artificial intelligence (AI), perceives its benefits, and reflects on limitations. Methods conducted a qualitative, systematic discourse analysis 200 social-media posts collected over period five months (April–August 2020). Results The AI is active, albeit ofte...

Context: COVID-19 has severely impacted traditional approaches to Practice Based Research recruitment strategies. The Center for Primary Care at Atrium Health is actively enrolling participants as a site PRagmatic EValuation of evENTs And Benefits Lipid-lowering in oldEr adults (PREVENTABLE), multi-center, decentralized trial. Objective: Pragmatic, trials are an effective way ...

Following acetylation, newly synthesized H3-H4 is directly transferred from the histone chaperone anti-silencing factor 1 (Asf1) to chromatin assembly factor 1 (CAF-1), another histone chaperone that is critical for the deposition of H3-H4 onto replicating DNA. However, it is unknown how CAF-1 binds and delivers H3-H4 to the DNA. Here, we show that CAF-1 binds recombinant H3-H4 with 10- to 20-f...

ABSTRACT Introduction Increasing rates of liver transplantation and improved outcomes have led to greater numbers transplant recipients followed up in non-transplant centres. Our aim was document long-term clinical managed this ‘hub-and-spoke’ healthcare model. Methods A retrospective analysis all adult patients who underwent between 1987 2016, with post-transplant fo...

Methylation of histone H3 at lysine 4 (H3 Lys-4) or lysine 9 (H3 Lys-9) is known to define active and silent chromosomal domains respectively from fission yeast to humans. However, in budding yeast, H3 Lys-4 methylation is also necessary for silent chromatin assembly at telomeres and ribosomal DNA. Here we demonstrate that deletion of set1, which encodes a protein containing an RNA recognition ...

Background: Because of the Coronavirus disease 2019 (COVID 19) pandemic, many primary care practices have transitioned to telehealth visits keep patients at home and decrease transmission disease. Yet, little is known about nationwide capacity for delivering services via telehealth. Methods: Using 2016 National Ambulatory Medical Survey we estimated number proportion reported ...