نتایج جستجو برای: gus gene
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The C1 promoter expressing the AC1 gene, and V1 promoter expressing the AV1 gene are located in opposite orientations in the large intergenic region of the Cotton leaf curl Burewala virus (CLCuBuV) genome. Agro-infiltration was used to transiently express putative promoter constructs in Nicotiana tabacum and Gossypium hirsutum leaves, which was monitored by a GUS reporter gene, and revealed tha...
Genes encoding phenyla]anine ammonia-tyase (PAL) form a small multigene family with at least three members in pea. Tissue-specific expression of the promoter of a member of PAL gene family (RSR4Ll) was inyestigated in the transgenic tobacco transformants earrying the different modes of chimeric fusion between the RSItaLl promoter and a bacteria] ll-glucuronidase (GUS) gene. In stems, at least, ...
In order to develop an easy, rapid and efficient protocol for the genetic transformation of tomato (Lycopersicon esculentum Mill.), without involving tissue culturing, ripened fruits of tomato were infiltrated with Agrobacterium harboring plasmid pROKII GUSint AP1. The plasmid contains Arabidopsis early flowering gene AP1, GUS reporter gene and NPTII as a selectable marker gene. Both transient ...
The Arabidopsis APETALA3 (AP3) and PISTILLATA (PI) proteins are thought to act as transcription factors and are required for specifying floral organ identities. To define the nuclear localization signals within these proteins, we generated translational fusions of the coding regions of AP3 and PI to the bacterial uidA gene that encodes beta-glucuronidase (GUS). Transient transformation assays o...
Investigations were conducted to determine whether induction of PR-1a gene promoter was correlated with systemic resistance, induced by rhizobacteria, against wildfire disease of tobacco. Ten different strains of plant growth-promoting rhizobacteria (PGPR), including the species Bacillus pumilus, Serratia marcescens, Pseudomonas fluorescens, P. putida, Curtobacterium flaccumfaciens, and Bukhold...
Cells of yellow-poplar (Liriodendron tulipifera L.) were transformed by direct gene transfer and regenerated into plants by somatic embryogenesis. Plasmid DNA bearing marker genes encoding beta-glucuronidase (GUS) and neomycin phosphotransferase (NPT II) were introduced by microprojectile bombardment into single cells and small cell clusters isolated from embryogenic suspension cultures. The nu...
We have produced 22 090 primary transgenic rice plants that carry a T-DNA insertion, which has resulted in 18 358 fertile lines. Genomic DNA gel-blot and PCR analyses have shown that approximately 65% of the population contains more than one copy of the inserted T-DNA. Hygromycin resistance tests revealed that transgenic plants contain an average of 1.4 loci of T-DNA inserts. Therefore, it can ...
BACKGROUND Promoters with tissue-specificity are desirable to drive expression of transgenes in crops to avoid accumulation of foreign proteins in edible tissues/organs. Several photosynthetic promoters have been shown to be strong regulators of expression of transgenes in light-responsive tissues and would be good candidates for leaf and immature fruit tissue-specificity, if expression in the ...
The regulatory region of the ribulose-1,5-bisphosphate carboxylase small subunit gene SRS4 from soybean (Glycine max) was cloned using TAIL-PCR and general PCR, and named the rbcS promoter. The promoter was fused with the GUS gene and introduced into Nicotiana tabacum via Agrobacterium-mediated leaf disk transformation. In 4-week-old transgenic tobacco plants, the highest GUS expression levels ...
The arcA, a member of the G protein beta-subunit family, was isolated from tobacco BY-2 cells as an auxin-responsive gene. Characterization of arcA, which should help to elucidate the function of the gene product in the plant cells, was performed with emphasis on the mode of expression and the analysis of its promoter. Accumulation of the arcA message was detected only after treatments with aux...
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