The remarkable ability of guanidinium chloride (GdmCl) to denature proteins is a well studied yet controversial phenomenon; the exact molecular mechanism is still debatable, especially the role of hydration dynamics, which has been paid less attention. In the present contribution, we have addressed the issue of whether the collective hydrogen bond dynamics of water gets perturbed in the presenc...

Bovine nasal cartilage was repeatedly extracted with the dissociative solvent, 4 M guanidinium chloride. About 25% of the total tissue proteoglycans could not be solubilized as judged by galactosamine analysis. The inextractable proteoglycan could be at least partially solubilized by treating the guanidine-extracted tissue with reagents which completely or partially degraded the proteoglycan pr...

We have investigated the effect of increasing sodium dodecyl sulfate (SDS) concentrations on rabbit muscle cytosolic creatine kinase structure by two methods. We have first determined the variation of accessibility of the thiol groups of the enzyme during SDS denaturation by a technique which involves an irreversible chemical modification of CK accessible thiol groups, followed by NTCB cleavage...

Proteoglycans were isolated from cartilage by extraction with 4M-guanidinium chloride followed by direct centrifugation in 4M-guanidinium chloride/CsCl at a low starting density, 1.34 g/ml. N-Ethylmaleimide was included in the extraction solvent as a precaution against contamination of proteoglycans with unrelated proteins mediated by disulphide exchange. A novel, discrete, low-buoyant-density ...

Revell & Muir (1972) have studied the metabolism of 35S-labelled proteoglycan from porcine cartilage after injection into guinea pigs. They concluded that the polymeric material excreted in the urine was the result of proteolytic degradation of the injected proteoglycan to single chains of chondroitin sulphate. These results are particularly interesting with respect to the site(s) and mechanism...

Previous physical studies from this laboratory by sedimentation velocity and ditfusion and by sedimentation equilibrium (YuE, R. H., RATLIFF, R. L., AND KWY, S. A. (1967) Biochemistry 6, 2923-2932) have permitted an assignment of the kinetic molecular weight of the crystalline brewers’ yeast nucleoside diphosphokinase, namely 102,000 ( 12,000) at infinite dilution. The observed concentration de...

Circular dichroism spectra for C3a anaphylatoxins (protein fragments generated enzymatically in serum during activation of the third component of complement (C3)) from human and porcine sources were compared in the region of 190 to 250 nm. The spectra were indistinguishable in this region, although an estimated difference of *approximately 20% exists between the primary structures of human and ...