This paper presents a novel spectrophotometric method to measure peroxidase activity using paraphenylenediamine dihydrochloride (PPDD) and Mequinol (MQ). The PPDD traps the free radical, and gets oxidized to electrophilic 1,4-diimine; this couples with MQ to an give intense violet-colored chromogenic species with the maximum absorbance at 560 nm. This assay was adopted for the quantification of...

Peroxidase (POD) associated with the browning of fresh-cut fruits and vegetableswas extracted from purple skin eggplant(Solanum melongena L.) characterised using reliable spectrophotometric methods. Maximal POD activity was found at 35 °C pH 6.0 guaiacol as substrate. The enzyme stable his optimal temperature (35 °C) hisat stability in range 5.6 - 6.6.Peroxidase retained its full presence ion...

The glutathione peroxidase-glutathione reductase system, an alternative pathway for metabolic utilization of H2O2 [Chance, Sies & Boveris (1979) Physiol. Rev. 59, 527-605], was investigated in Trypanosoma cruzi, an organism lacking catalase and deficient in peroxidase [Boveris & Stoppani (1977) Experientia 33, 1306-1308]. The presence of glutathione (4.9 +/- 0.7 nmol of reduced glutathione/10(8...

Plant (Class III) peroxidases have numerous applications throughout biotechnology but their thermal and oxidative stabilities may limit their usefulness. Horseradish peroxidase isoenzyme C (HRPC) has good catalytic turnover and is moderately resistant to heat and to excess (oxidizing) concentrations of hydrogen peroxide. In contrast, HRP isoenzyme A2 (HRP A2) has better oxidative but poorer the...

Wild-type recombinant horseradish peroxidase purified and refolded from Escherichia coli inclusion bodies has been studied in the system of bis(2-ethylhexyl)sulphosuccinate sodium salt (Aerosol OT)-reversed micelles in octane. In contrast with native horseradish peroxidase the wild-type recombinant enzyme forms dimeric structures as judged by sedimentation analysis. Peroxidase substrates affect...

The genomes of three representative Polyporales (Bjerkandera adusta, Phlebia brevispora and a member of the Ganoderma lucidum complex) were sequenced to expand our knowledge on the diversity of ligninolytic and related peroxidase genes in this Basidiomycota order that includes most wood-rotting fungi. The survey was completed by analyzing the heme-peroxidase genes in the already available genom...

Rare earth elements have been tested in control of plant diseases. Lanthanum (La) was the Alternaria solani (in vitro) and tomato early blight vivo) using concentration 0; 0.1; 0.2; 0.4 0.8 g L-1. In vitro, were diluted V8 culture medium evaluated for mycelial growth rate index (MGRI) pathogen sporulation. vivo, 24 hours after application inoculate inoculation, leaflets collected quantification...

Application of nanotechnology is crucial for a sustainable increase in food production to cope with the increasing demand burgeoning population. Wheat has significantly security Pakistan help nanotechnology. In biological systems, utilization nanoparticles been increased due their growth-promoting effects on germination, photosynthetic attributes, nutrient use efficiency and metabolic activitie...

Aluminum toxicity is one of the most deleterious factors for plant growth in acidic soils because over 50% of the world’s potentially arable lands are acidic. In recent years, considerable research has been conducted to understand the mechanisms of Al toxicity and tolerance in plants. This paper reviews the effects of different concentration of Al on plant shoot, root physiological parameters s...