Piscine iridoviruses infect a wide variety of fish and are classified into three genera: Lymphocystivirus, Ranavirus and Megalocytivirus. Lymphocystiviruses cause non-fatal, dermal infections, while ranaviruses and megalocytiviruses produce devastating, systemic infections with mortality reaching up to 100%. Although both ranaviruses and megalocytiviruses cause fatal systemic infections, they i...

Backcross breeding between TGGG hybrid grouper (Epinephelus fuscoguttatus  E. lanceolatus) and giant (E.

A red grouper was caught with a spear gun at depths of 8-10 m the Alanya Harbor Breakwater. The purpose this study is to determine species, which seen for first time in Mediterranean coasts Turkey, and add it Gulf Antalya fish fauna. In species identification, determined that Epinephelus fasciatus. E. fasciatus second record Turkish coast. This became lessepsian Serranid detected Antalya, numbe...

Major histocompatibility complex (MHC) molecules play vital roles in triggering adaptive immune responses and are considered the most variable molecules in vertebrates. Recently, many studies have focused on the polymorphism and evolution mode of MHC in both model and non-model organisms. Here, we analyzed the MHC class II exon 2-encoding β chain in comparison with the mitochondrial Cytb g...

Stress-activated protein kinases (SAPKs), including p38 mitogen-activated protein kinase (p38 MAPK) and c-Jun N-terminal kinase (JNK), are usually activated in response to different environmental stimuli, including virus infection. In the present study, the roles of SAPKs during Singapore grouper iridovirus (SGIV) infection were investigated in fish cells. The results showed that increased phos...

Singapore grouper iridovirus (SGIV), a major pathogen of concern for grouper aquaculture, has a dsDNA genome with 162 predicted open reading frames, of which a total of 62 SGIV proteins have been identified. One of these, ORF158L, bears no sequence homology to any other known protein. Knockdown of orf158L using morpholino antisense oligonucleotides resulted in a significant decrease of virus yi...

Viral nervous necrosis (VNN), caused by a fish nodavirus, is one of the most serious fish diseases worldwide. Here we report a unique vaccination method in sevenband grouper Epinephelus septemfasciatus using a synthetic double-stranded RNA polyinosinic polycytidylic acid (Poly(I:C)), an interferon inducer, followed by challenge with a live fish nodavirus. Fish injected with Poly(I:C) at 200 mic...

Interferon regulatory factor 2 (IRF-2) is a multifunctional transcription factor which exhibits both transcriptional activating and repressing activities in the IRF family. In this study, we report an IRF-2 gene isolated from orange-spotted grouper (Epinephelus coioides). The 1854bp full-length cDNA sequence of EcIRF-2 has been cloned, encoding a putative peptide of 336 amino acids which is hig...

The Atlantic Goliath Grouper, Epinephelus itajara, is critically endangered throughout its range but has begun to show initial signs of recovery in Florida state waters. As the population continues to rebound, researchers face a pressing need to fill the knowledge gaps about this iconic species. Here, we examined the δ (15)N isotopic records in fin rays collected from Atlantic Goliath Grouper, ...