نتایج جستجو برای: granzymes

تعداد نتایج: 1452  

Journal: :The Biochemical journal 2011
Stefanie A H de Poot Marijn Westgeest Daniel R Hostetter Petra Van Damme Kim Plasman Kimberly Demeyer Roel Broekhuizen Kris Gevaert Charles S Craik Niels Bovenschen

Cytotoxic lymphocyte protease GrM (granzyme M) is a potent inducer of tumour cell death and a key regulator of inflammation. Although hGrM (human GrM) and mGrM (mouse GrM) display extensive sequence homology, the substrate specificity of mGrM remains unknown. In the present study, we show that hGrM and mGrM have diverged during evolution. Positional scanning libraries of tetrapeptide substrates...

Journal: :Cell 2003
Debabrata Chakravarti Rui Hong

Cytotoxic T lymphocytes release granzymes (Gzm) A and B to induce apoptosis or programmed cell death of virally infected or tumor cells. In this issue of Cell, Fan et al. identify the tumor metastasis suppressor NM23-H1 as a GzmA-activated, apoptosis-inducing DNase and the oncoprotein SET as its inhibitor. Work from the Lieberman and Wang groups indicates a surprising role for a group of acidic...

Journal: :Journal of immunology 1999
J P Galvin L H Spaeny-Dekking B Wang P Seth C E Hack C J Froelich

Lymphocyte granule-mediated apoptosis occurs by perforin-mediated intracellular delivery of granule-associated serine proteases (granzymes). A granule-associated proteoglycan, namely serglycin, that contains chondroitin 4-sulfate (CS) glycosaminoglycans is present in the granules of cytotoxic cells. Serglycin acts as scaffold for packaging the positively charged granzymes and probably chaperone...

Journal: :Angewandte Chemie 2023

Cytotoxic immune cells, including T lymphocytes (CTLs) and natural killer (NK) are essential components of the host response against tumors. CTLs NK cells secrete granzyme A (GzmA) upon recognition cancer cells; however, there very few tools that can detect physiological levels active GzmA with high spatiotemporal resolution. Herein, we report rational design near-infrared fluorogenic substrate...

Journal: :Proceedings of the National Academy of Sciences of the United States of America 2011
Tilen Praper Andreas F-P Sonnen Ales Kladnik Alberto O Andrighetti Gabriella Viero Keith J Morris Emanuela Volpi Lorenzo Lunelli Mauro Dalla Serra Christopher J Froelich Robert J C Gilbert Gregor Anderluh

The cytotoxic cell granule secretory pathway is essential for immune defence. How the pore-forming protein perforin (PFN) facilitates the cytosolic delivery of granule-associated proteases (granzymes) remains enigmatic. Here we show that PFN is able to induce invaginations and formation of complete internal vesicles in giant unilamellar vesicles. Formation of internal vesicles depends on native...

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