A central problem of the current studies on protooncogenes (cellular oncogenes, c-onc) is their role in the control of growth and differentiation of normal cells. The cfos protooncogene has been widely investigated and it has been demonstrated that cfos specific transcripts are induced in proliferating cells (1, 2) and during differentiation (3-6) . In particular, when HL60 (promyelocytic) and ...

The effect of purified recombinant human granulocyte-macrophage colony-stimulating factor (GM-CSF) on the oxidative metabolism of human peripheral blood granulocytes was investigated. The respiratory burst of granulocytes was assessed in individual cells by flow cytometry utilizing the oxidation of the nonfluorescent 2',7'-dichlorofluorescein (DCFH) to the highly fluorescent DCF by hydrogen per...

P REVIOUS STUDIES of granulocyte kinetics in hematologically normal individuals employing tritiated thymidine ( H3TdR) have indicated that there is a 4-5 day period after administration of the radioisotope before labeled granulocytes appear in the peripheral blood.13 The large mass of non-proliferating granulocytic cells constitutes the marrow granulocyte reserve (MGR) from which the majority o...

Proximal femur fracture, a typical fracture of the elderly, is often associated with morbidity, reduced quality of life, impaired physical function and increased mortality. There exists evidence that responses of the hematopoietic microenvironment to fractures change with age. Therefore, we investigated oxidative stress markers and oxidative stress-related MAPK activation in granulocytes from t...

In a previous publication from this laboratory (1), radioactive diisopropylfluorophosphate (DFP32), a potent and irreversible esterase inhibitor, was shown to be a suitable label for granulocytes. Evidence was presented that the label is firmly attached to granulocytes; it does not modify the viability of the cells, and the label is not reutilized when the cells are degraded. Experiments design...

Both asthmatic and COPD patients were found to have increased amounts of granulocytes and matrix metalloproteinase-9 (MMP-9) in their sputum. The present study was conducted to investigate whether the elevated amounts of MMP-9 and TIMP-1 found in such patients' airways may be linked to an enhanced secretion by granulocytes. Blood granulocytes from asthmatics (n = 10), COPD patients (n = 11), an...

Unlike unanimous opinion on tissue factor (TF) expression in monocytes, the quest for TF presence in granulocytes has been going on for decades. To study the cell origin and track the blood-borne TF, we assessed TF activity and protein levels, knocked-down endogenous TF expression with small interfering RNA (siRNA), and overexpressed TF-yellow fluorescent protein (TF-YFP) fusion in immunologica...

OBJECT We sought to detect an acute soft tissue infection in rats by magnetic resonance imaging (MRI) using granulocytes, previously labeled with superparamagnetic particles of iron oxide (SPIO). MATERIALS AND METHODS Parasternal infection was induced by subcutaneous inoculation of Staphylococcus aureus suspension in rats. Granulocytes isolated from isogenic donor rats were labeled with SPIO....

Voltage-gated proton current (I(Hv)) has been characterized in several cell types, but the majority of the data was collected in phagocytes, especially in human granulocytes. The prevailing view about the role of I(Hv) in phagocytes is that it is an essential supporter of the intense and sustained activity of Nox2 (the core enzyme of the phagocyte NADPH oxidase complex) during respiratory burst...

Effects of fetal bovine serum (FBS) and complement on phagocytic activity in Ornithodaros moubata (Murray 1877) hemocytes and protease activity in the hemocytes were examined. At least three morphologically different cell types, granulocytes, plasmatocytes, and prohemocytes, were detected in hemolymph of O. moubata, and granulocytes and plasmatocytes showed phagocytic activity. FBS altered phag...