During differentiation in a thymus, T lymphocytes learn to recognize self alloantigens determined by the H-2 major histocompatibility complex. This imposes certain genetic restrictions on lymphocyte interaction during T cell-mediated immune responses (1-3). For example, antigen-specific helper T cells acquire a binding capacity not only for a given foreign antigen but also for Ia alloantigens o...

Persistent stimulation of immunocompetent cells, such as histocompatibility reactions, may be one of the causes of neoplastic proliferation of lymphoreticular tissues. In the present studies, the graft-versus-host reaction was induced in 18week-old male ( C 57 Bl/6] x DBA/2 J )F1 mice · by two or four weekly intraperitoneal injections of approximately 80 x 106 male C 57 Bl/6 J or C 57 Bl/Na mou...

Inbred mice were inoculated with transplantable leukemia. X-irradiated, and given injections of bone marrow and spleen cells from allogeneic donors. Control animals received syngeneic cells after irradiation. On Days 1 to 7 after this treatment, the mice were individually tested for the presence of leukemic cells by transferring from each the spleen, a piece of liver, left kidney, left lung, bl...

T cell clones isolated from class II MHC-disparate MLR combinations, and specific for I-Ak and I-Ek molecules, respectively, are shown to induce acute lethal graft-vs-host disease in unirradiated recipients. Cytolytic and noncytolytic clones are equally efficient in this respect. The lethal disease is dependent on recognition of the stimulatory class II molecules in the host. The clones home to...

Thymectomy of Sprague-Dawley rats on the 3rd day of life failed to influence the time of onset, incidence, clinical, or histologic picture of runt disease produced by the intraperitoneal injection of adult Long-Evans spleen cells. The fact that severe immunologic impairment of the host by thymectomy does not modify runt disease was felt to be consistent with the current view that the if directi...

Gribben JG, Freedman AS, Neuberg D, et al. Immunologic purging of marrowassessed by PCRbefore autologous bone marrowtransplantation for B-cell lymphoma. N Engl J Med 325: 1525, 1991. HamonMD, Prentice HG, Gottlieb DJ, et al. Immunotherapy with interleukin 2 after ABMTin AML.Bone Marrow Transplant ll: 399, 1993. Yanai N, Sekine C, Yagita H, Obinata M, Roles for integrin vary late activation anti...

The effect of ubenimex on graft-versus-host reaction and delayed cutaneous hypersensitivity in mice was studied. Ubenimex enhanced the graft-versus-host reaction in a dose range from 0.005 to 0.5 mg/kg. Ubenimex inhibited the aging-caused decrease of delayed cutaneous hypersensitivity to oxazolone and the efficacy was greater with older mice. Ubenimex also inhibited mitomycin C and L1210-caused...

Adult CBA mice may be made tolerant of August rat tail skin grafts by short intensive treatments with antilymphocyte serum followed by massive ( approximately 100 x 10(6) cells) injections of August rat lymphoid cells. Prolonged tolerance can be achieved only in thymectomized mice, and is accompanied by lymphoid cell chimerism and the manufacture of rat protein. The rat lymphoid cell donors mus...

In this study we have investigated whether addition of bone marrow accessory cells or concurrent administration of recombinant IL-2 would allow intraepithelial lymphocytes (IEL) to induce a systemic, lethal GvHR in irradiated hosts. In addition we have studied the ability of IEL to migrate into lymphoid tissues after intravenous injection and compared this with their locomotor capacity in vitro.