The isolation and sequence of a cDNA clone encoding the complete glyoxysomal malate dehydrogenase [gMDH; (S)-malate:NAD+ oxidoreductase, EC 1.1.1.37] of watermelon cotyledons are presented. Partial cDNA clones were synthesized in a three part strategy, taking advantage of the polymerase chain reaction technology with oligonucleotides based on directly determined amino acid sequences. Subsequent...

We have studied the significance of the N-terminal presequence of watermelon (Citrullus vulgaris) glyoxysomal malate dehydrogenase [gMDH; (S)-malate:NAD+ oxidoreductase; EC 1.1.1.37] in microbody targeting. The yeast Hansenula polymorpha was used as heterologous host for the in vivo expression of various genetically altered watermelon MDH genes, whose protein products were localized by immunocy...

This paper develops a novel approach to incorporate the contributions of both quantitative validation metrics and qualitative subject matter expert (SME) evaluation criteria in model validation assessment. The relationship between validation metrics (input) and SME scores (output) is formulated as a classification problem, and a probabilistic neural network (PNN) is constructed to execute this ...

Objective: In general, financial time series such as stock indexes have nonlinear, mutable and noisy behavior. Structural and statistical models and machine learning-based models are often unable to accurately predict series with such a behavior. Accordingly, the aim of the present study is to present a new hybrid model using the advantages of the GMDH method and Non-dominated Sorting Genetic A...

PURPOSE The specialized corneal epithelium requires differentiated properties, specific for its role at the anterior surface of the eye. Thus, tight maintenance of the differentiated qualities of the corneal epithelial is essential. Pinin (PNN) is an exon junction component (EJC) that has dramatic implications for corneal epithelial cell differentiation and may act as a stabilizer of the cornea...

PURPOSE Pinin (Pnn/DRS/memA) is a cell-adhesion-related and nuclear protein that has been identified as central in the establishment and maintenance of corneal epithelial cell-cell adhesion. To begin the elucidation of the role of Pnn within the nucleus of corneal epithelial cells, this study was undertaken to identify the proteins that bind to Pnn. METHODS Yeast two-hybrid analyses were perf...

A new methodology for Emergent System Identification is proposed in this paper. The new method applies the self-organizing Group Method of Data Handling (GMDH) functional networks, Particle Swarm Optimization (PSO), and Genetic Programming (GP) that is effective in identifying complex dynamic systems. The focus of the paper will be on how Particle Swarm Optimization (PSO) is applied within Grou...

Age-related changes in inhibitory neurotransmission in sensory cortex may underlie deficits in sensory function. Perineuronal nets (PNNs) are extracellular matrix components that ensheath some inhibitory neurons, particularly parvalbumin positive (PV+) interneurons. PNNs may protect PV+ cells from oxidative stress and help establish their rapid spiking properties. Although PNN expression has be...

An Artificial Neural Network is a flexible mathematical structure which is capable of identifying complex nonlinear relationships between input and output data sets. Such Neural Networks have been characterized by passive neurons that are not able to select and estimate their own inputs. In a new approach, which corresponds in a better way to the actions of human nervous system, the connections...