Metabolic enzymes have been known to carry out a variety of functions besides their normal housekeeping roles known as "moonlighting functions." These functionalities arise from structural changes induced by posttranslational modifications and/or binding of interacting proteins. Glycolysis is the sole source of energy generation for malaria parasite Plasmodium falciparum, hence a potential path...

The objective of this study was to determine the relationships between glycolytic enzyme activities and metabolite contents measured at 45 min postmortem in porcine longissimus dorsi muscle, and also investigated the influence of the glycolytic traits on meat quality. The activities of lactate dehydrogenase (LDH) and pyruvate kinase (PK) were correlated with ATP content, and LDH activity was po...

Classically viewed as a cytosolic pathway, glycolysis is increasingly recognized as a metabolic pathway exhibiting surprisingly wide-ranging variations in compartmentalization within eukaryotic cells. Trypanosomatid parasites provide an extreme view of glycolytic enzyme compartmentalization as several glycolytic enzymes are found exclusively in peroxisomes. Here, we characterize Trypanosoma bru...

BACKGROUND Recent studies in animal models, based on the hypothesis that malignant glioma cells are more dependent on glycolysis for energy generation, have shown promising results using ketogenic diet (KD) therapy as an alternative treatment strategy for malignant glioma, effectively starving glioma cells while providing ketone bodies as an energy source for normal neurons and glial cells. In ...

Glycolysis (Embden-Meyerhof-Parnas pathway) is the most common sequence of reactions for the conversion of glucose-6-P into pyruvate in all domains of life. It generates ATP, reduced equivalents, and precursor metabolites for a multitude of essential cellular processes. During growth on substrates other then hexoses, essential glycolytic intermediates are synthesized via glyconeogenesis, revers...

A transition from aerobic to anaerobic metabolism occurs as mitogen-activated thymocytes undergo proliferation. Glucose utilization and lactate formation increases 18- and 38-fold, respectively, during proliferation. The absolute amount of 14CO2 production by pyruvate dehydrogenase remains constant, while 14CO2 production by the tricarboxylic acid cycle is reduced during transition from a resti...

The kinetics of induced synthesis of glycolytic enzymes of the hybrid yeast Saccharomyces fragilis X Saccharomyces dobzhansizii in response to the addition of glucose or galactose has been studied in a basal medium containing peptone, acetate, and yeast extract. Glucose and galactose bring about a 3to loo-fold increase in specific activity of various glycolytic enzymes during a ‘Thour period. T...

This study evaluated the link between swimming endurance and condition of Atlantic cod Gadus morhua that had been fed or starved during the 16 weeks preceding the tests, and assessed whether muscle metabolic capacities explain such links. The condition factor [(somatic mass x fork length(-3))x100] of starved cod was 0.54+/-0.1 whereas that of fed cod was 0.81+/-0.1. In white and red muscle, we ...

Growth temperature has a profound impact on the kinetic properties of enzymes in microbial metabolic networks. Activities of glycolytic enzymes in Saccharomyces cerevisiae were up to 7.5-fold lower when assayed at 12 degrees C than at 30 degrees C. Nevertheless, the in vivo glycolytic flux in chemostat cultures (dilution rate: 0.03 h(-1)) grown at these two temperatures was essentially the same...