Lipases (triacyl glycerol acylhydrolases, EC 3.1.1.3) belonging to the class Hydrolases catalyze hydrolysis of insoluble triacylglycerols (TAGs) to generate free fatty acids, mono and diacylglycerols and glycerol (Das et al., 2016). Lipases are found in both prokaryotes (bacteria and archaea) as well as in eukaryotes (animals, plants and fungi) (Cai-hong et al., 2008). The most broadly applied ...

This study was aimed to determine the acylglycerols composition, quality characteristics, and protective role of dietary pumpkin seed oil (PSO) in rabbits. PSO was thermally oxidized and analyzed for quality characteristics and acylglycerols composition using reversed phase high performance liquid chromatography with diode array detection (HPLC-DAD). Oxidized and un-oxidized oil samples were fe...

background : this study was set to determine the optimal technique of breast cancer with supraclavicular fossa irradiation. methods : three techniques was compared in this study. techniques were two point symmetric (2p-sym), two point asymmetric (2p-asym) and single point (sp). in 2p-sym all of beams were symmetric, in 2p-asym tangential beams were symmetric and supra was asymmetric, in sp all ...

Non-covalent molecular imprinting of poly(allylamine hydrochloride) (PAA HCl) with glucose phosphate mono-sodium salt produced molecularly imprinted polymer (MIP) hydrogels capable of quantitative, isomerically specific binding of glucose. By ionic association of a template molecule, glucose phosphate mono-sodium salt, to the polymer prior to covalent crosslinking, MIP hydrogels were created wi...

A triacylglycerol lipase, presumably the first enzyme involved in the mobilization of lipid from the insect fat body, has been purified to homogeneity from the fat body of Manduca sexta. The purification procedure involved polyethyleneglycol precipitation, and chromatography on DEAE-cellulose, phenyl-Sepharose, Q-Sepharose and hydroxylapatite. The final product, a protein with an M(r) = 76,000 ...

The European Standard EN 14105:2011-07 is an analysis method for quantifying free glycerol and residual mono-, di- and triacylglycerides impurities in biodiesel by gas chromatography. The method specifies an "on-column injector or equivalent device" as the means of sample introduction. Cool on-column (COC) would appear to be an ideal choice, particularly for quantifying triacylglycerides, as it...

The galactose, glycerol, and fatty acids of mono- and digalactosyl diglycerides (MGDG and DGDG) have been separated and analyzed for (14)C activity after (14)CO(2) feeding of Vicia faba leaf discs. Fully expanded and developing leaves were analyzed at time intervals following feeding during continuous illumination. In addition, fully expanded leaves were analyzed after similar times in complete...

CITREM is an emulsifier used in the food industry and contains citric acid esters of mono- and diglycerides (GCFE). It is generally recognized as safe but no publication on its digestibility under gastrointestinal conditions and impact on fat digestion was available. It was shown here that fatty acids are released from CITREM by gastric lipase, pancreatic lipase, pancreatic-lipase-related prote...

Changes in the release and esterification of free fatty acids (FFA) in adipose tissue were looked for as a cause of moderate primary hypertriacylglycerolemia (HTG) in five obese subjects. Comparison was made with six obese normolipidemic subjects. The two groups were matched for body weight, tolerance of intravenous glucose, fat cell size, fasting levels of serum immunoreactive insulin, and ser...