The marked stimulatory effect of insulin on the conversion of 20 mM D-[6-14C]glucose to CO2, glyceride-glycerol, and fatty acid observed in small rat adipocytes was greatly diminished in large cells from older rats. Similarly, total glucose utilization as estimated by summing the total metabolites accumulated intracellularly plus the release of labeled CO2 and lactate was substantially lower in...

When rat livers were perfused with red cell-albumin solutions containing high concentrations of free fatty acids (FFA), the FFA were very rapidly taken up. Concomitantly, the glyceride content of the liver rose by 25-60% over control values, the absolute increment corresponding roughly to the amount of FFA taken up. The net increase in liver glyceride content was independent of whether palmitat...

It has been shown by several workers that fatty acids liberated by the hydrolyses of their esters, such as amyl (I), ethyl (2), or the optically active mannite (3), appear in the thoracic duct chyle as triglycerides. In comparing the absorption of free palmitic acid and some of its esters, Lyman (4) found that in each case the fat deposited in the tissues was tripalmitin. It was assumed, theref...

Segments of epididymal adipose tissue from normal male rats were incubated with micromolar concentrations of colchicine for different periods of time up to 4 hr, and the mobilization of free fatty acids (FFA) was measured during a subsequent reincubation. Although pretreatment with colchicine did not alter basal unstimulated FFA release, mobilization of FFA in the presence of epinephrine or the...

Factors involved in the hyperlipidemia of nephrosis have been studied in seven patients. The turnover of triglyceride was measured in plasma very low density lipoproteins after the injection of glycerol-(14)C. The turnover of esterified cholesterol was measured in whole plasma and in very low density lipoproteins after the injection of mevalonic acid-2-(3)H. Urine protein loss was found to be s...

1. 0.5mm-Palmitate stimulated incorporation of [U-14C]glucose into glyceride glycerol and fatty acids in normal fat cells in a manner dependent upon the glucose concentration. 2. In the presence of insulin the incorporation of 5mM-glucose into glyceride fatty acids was increased by concentrations of palmitate, adrenaline and 6-N-2'-O-dibutyryladenosine 3':5'-cyclic monophosphate up to 0.5mM, 0....