1. The basic glutathione S-transferases from rainbow-trout liver were more stable than the acidic ones. 2. The apparent pI values of these enzymes were lowered when they were eluted from a glutathione affinity column by reduced glutathione at pH 8.85. 3. The pI effect was not a function of the high pH alone, was diminished under conditions less favourable to glutathione oxidation, and did not o...

The activity of eleven enzymes involved in development, ageing and in metabolism of xenobiotics in insects, was investigated under the influence of 2,4-D (2,4-Dichlorophenoxyacetic acid), in the mustard aphid, Lipaphis erysimi (Kaltenbach) (Rhynchota Aphididae), after treating the 2 instar nymphs for 13, 25 and 37 h. Studies of two hydrolases and one group transfer enzyme revealed significant e...

A purification scheme was devised that resulted in the resolution of a number of basic glutathione S-transferases from rat liver, three of which contained two subunits of molecular mass 23500 Da (i.e. Yb monomers). These were identified as transferases D, C and A by their elution positions from CM-cellulose and their specific activities towards a variety of substrates. Hybridization, immunotitr...