A new method for one-pot synthesis of 2,3-substituted piperidine from N-PMP aldimine and aqueous glutaraldehyde via formal [4+2] cycloaddition is reported. This reaction involves organocatalytic direct Mannich reaction-reductive cyclization with high yields (up to 90%) and excellent enantioselectivities (up to >99%). The practicability of this method is also shown at a gram scale as well as thr...

Super-resolution microscopy (SRM) allows precise localization of proteins in cellular organelles and structures, including the actin cytoskeleton. Yet sample preparation protocols for SRM are rather anecdotal and still being optimized. Thus, SRM-based imaging of the actin cytoskeleton and associated proteins often remains challenging and poorly reproducible. Here, we show that proper paraformal...

Poly(vinyl alcohol) and poly(vinyl pyrrolidone) were blended and films were obtained by casting method. Glutaraldehyde, hydrochloric acid and lactic acid were added to investigate their effect on mechanical properties, swelling and solubility of the blends with the aim to find a possible material candidate for medium or long term implants. Combination of all three additives (lactic acid, glutar...

The ability of glutaraldehyde-treated tumor cells to induce protection against subsequent challenge has been studied in a syngeneic system. Two tumors have been tested in BALB/c mice. The first was a methylcholanthreneinduced tumor that has been maintained in serial passage for over a decade. The second was a spontaneous mammary adenoacanthoma that was tested at the third passage. The protectio...

Glutaraldehyde is a water-soluble liquid with high bactericidal, sporicidal, fungicidal and viricidal activity. It does not cross-react with formaldehyde. Glutaraldehyde solution is an underutilized gem for treating recalcitrant palmar, plantar and periungual warts. It has also been utilized for plantar hyperhidrosis with moderate success, for pitted keratolysis and the predisposing factor of h...

The extraction of the exopolymeric matrix is a prerequisite to properly assessing the composition of the biofilm. Several extraction methods have already been developed, however, no universal method has yet been adopted because the compromise between high yields of extraction and minimum cell lysis is difficult to establish. In fact, most of the extraction methods promote leakage of intracellul...

E CRUMPLING of disc-shaped erythrocytes into bizarre forms is considered the basic pathological feature of sickle cell anemia.1 Several investigators have established that erythrocyte sickling is due to intracellular precipitation of deoxygenated sickle hemoglobin ( Hb S ).2.T A recent ultrastructural study by Murayarna identified the presence of tubular rods of Hb S in whole mount preparations...

The freeze-cleave technique demonstrates the presence of gap junctions at early stages of mouse cardiac muscle ontogenesis. The formation and growth of these junctions were studied at 4 stages of development: 10, 14, 18 days post-coitum (dpc) and at the adult stage. The diverse aspects of the gap junctions are interpreted as different steps in their formation. The first indication of this forma...

Peroxidatic activity of catalase (E .C .1 .11 .1 .6) was discovered by Keilin and Hartree (1), and it has been firmly established that, catalase oxidizes various substrates such as ethanol, methanol, nitrite, and formate (2-4) . Crystalline beef liver catalase, used as an enzyme tracer (5), and the peroxisomal catalase (6, 7) have been visualized cytochemically by a modification of the 3,3'diam...

Si/SiO(2) chips were used to detect the hybridization of immobilized Oligo d(T)(20) through impedance measurement. The immobilization procedure involved an aminopropyl silane grafted silicon oxide surface activated by glutaraldehyde and subsequently modified by an aminolinker supporting oligonucleotides. The immobilization procedure was optimized and, in the best conditions, the hybridization o...