the endotoxin dose response of recruited and activated tissue macrophages isolated from a rat model of liver injury has been measured after 24 hours in in vitro culture, with respect to their production of a lysosomal enzyme, n-acetyl-glucosaminidase (tlag). there were no significant differences in the nag activity in cell lysates or supernatants following a two-hour exposure of the macrophages...

Siastatins A and B were isolated as part of a program designed to find Streptomyces-produced inhibitors of sialidase from Clostridium perfringens. Siastatin A was more effective than was siastatin B in the inhibition of sialidases prepared from Cl. perfringens and chicken chorioallantoic membrane. However, siastatin B was a stronger inhibitor of sialidases prepared from streptomyces and rat org...

(8, 14-16). Earlier studies demonstrated differences in the activity of lysosomal enzymes of cells obtained from peritoneal dialysate effluent as compared to cells obtained from patients with normal renal function (17, 18). The present studies were aimed at the assessment of the effect of intermittent peritoneal dialysis on the activity of acid phosphatase (AP), beta-glucuronidase (GR), N-acety...

An endo-N-acetyl-glucosaminidase which degrades the murein (peptidoglycan) sacculi of the cell walls of Escherichia coli and Spirillum serpens, but not those of Micrococcus lysodeikticus and Sarcina lutea, is present as a contaminant in a "phospholipase C" from Clostridium perfringens. The specificity of enzyme action was elucidated by reduction of liberated glycosidic groups with NaBH4 and ide...