To determine the effect of increased glycogen stores on hepatic carbohydrate metabolism, 15 nondiabetic volunteers were studied before and after 4 d of progressive overfeeding. Glucose production and gluconeogenesis were assessed with [2-3H] glucose and [6-14C] glucose (Study I, n = 6) or [3-3H] glucose and [U-14C]-alanine (Study II, n = 9) and substrate oxidation was determined by indirect cal...

Biological diversity particularly at molecular level is astounding and can be used for rational manipulation of biological organisms. To analyze molecular diversity in its full scope computational models of biological organisms and biochemical pathways are indispensable. Engineering Sciences can be of great help in construction of these models. The central feature of modern engineering has been...

In vitro Evaluation of Gluconeogenesis Abstract The liver and kidneys maintain the blood glucose levels in periods of fasting, starvation, low-carbohydrate diets, or intense exercise by gluconeogenesis. The present study was designed to evaluate whether small intestine provides substrates for hepatic gluconeogenesis during fasting in an in-vitro model using rat everted sac technique. Healthy ad...

1. The activities at 15" of three gluconeogenic enzymes, D-fructose-l,6-diphosphate, 1-phosphohydrolase (EC 3.1.3.1 l), pyruvate carboxylase (EC 6.4.1.1) and phosphoenolpyruvate carboxykinase (4.1.1.32), were determined in liver, kidney, gill and muscle of rainbow trout (Sulmo guirdneri) given a commercial diet. The results indicated that liver and kidney are the main sites of gluconeogenesis. ...

The rate of appearance (Ra) of glucose in plasma and the contribution of gluconeogenesis were quantified in normal pregnant women early ( approximately 10 wk) and late ( approximately 34 wk) in gestation. Their data were compared with those of normal nonpregnant women. Glucose Ra was measured using the [U-13C]glucose tracer dilution method. Gluconeogenesis was quantified by the appearance of 2H...

Considerable data have accumulated over the past 20 years, indicating that the human kidney is involved in the regulation of glucose via gluconeogenesis, taking up glucose from the circulation, and by reabsorbing glucose from the glomerular filtrate. In light of the development of glucose-lowering drugs involving inhibition of renal glucose reabsorption, this review summarizes these data. Medli...

This paper reports experimental results that show that lysine at low concentrations can accelerate hepatic gluconeogenesis from lactate. The effect is catalytic. Although the mechanism of action of lysine is still unknown it is probable that lysine plays an important role in the regulation of liver metabolism. The lysine effect was discovered in the course of experiments on histidine metabolism...

To delineate the potential role of disordered glucose and glucose-precursor kinetics in the abnormal carbohydrate metabolism of chronic renal failure, alanine and glucose production and utilization and gluconeogenesis from alanine were studied in patients with chronic compensated renal insufficiency and in normal volunteers. With simultaneous primed injection-continuous infusions of radiolabele...