Cotransformation of Glomerella graminicola was achieved with the G. graminicola genes TUB1R1 (encoding a beta-tubulin which confers resistance to the fungicide benomyl) and PYR1 (encoding orotate phosphoribosyl transferase, which confers pyrimidine prototrophy). The cotransformation frequency was about 30% when selection was for pyrimidine prototrophy (Pyr) and 87% when selection was for benomy...
