BACKGROUND & OBJECTIVES Making centrifuged deposit smears from sputum to detect acid-fast bacilli (AFB) is considered hazardous. We carried out this study to stain the centrifuged deposits with carbol-fuchsin in sputum containers and to decolourize and counterstain their smears made on glass slides. METHODS The centrifuged deposits of 180 sputum samples from pulmonary tuberculosis patients we...

Automated image analysis of histology images is affected by the staining variations in histology slides. In general, training images are used to optimize the parameters of an image analysis system. Color, being one of the dominant features of stained tissue samples, is being commonly utilized as feature to segment or classify the different stained tissue components. However, the colors impresse...

Scoring micronuclei in the peripheral blood lymphocytes of individuals exposed to ionizing radiation is a rapid biodosimetry assay. Peripheral blood lymphocytes from five individuals were exposed in vitro to 0-5Gy of (60)Co γ-radiation at a dose rate of 0.76Gy/min. The blood cultures were initiated with RPMI-1640 (80%) supplemented with FBS (20%), stimulated with mitogen and incubated at 37°C f...

The evaluation of the morphology and organization collagen fibers is critical in understanding wound healing tissue remodeling after a thermal injury skin. However, histological analysis conducted by pathologists often labor-intensive limited to qualitative evaluations scoring within narrow field view. In this study, we propose convolutional neural network (CNN) model classify Masson's trichrom...

Background: Different histochemical stains have been applied to demonstrate the cytotoxic and genotoxic effects of cigarette smoking on cells. Feulgen Papanicolaou were most popular nuclear abnormalities. The aim this study was compare in demonstrating exfoliated oral mucosa Methods: A total 31 smokers 15 non-smokers included case-control study. Using a wooden spatula, two samples taken from ea...

AIM To determine the interobserver variation in scoring presence and grade of vulvar intraepithelial neoplasia (VIN) in haematoxylin/eosin (H/E) slides, MIB 1 slides, and the combined use of H/E and MIB 1 slides. METHODS 10 slides were stained with H/E and MIB 1 with each of the following diagnoses: normal vulvar skin, VIN 1, VIN 2, and VIN 3. Six observers first scored the H/E slides separat...

Cutaneous leishmaniasis (CL) in Morocco is caused by three species, Leishmania major, L. tropica and L. infantum. CL has been known in Chichaoua province since 2000. Using DNA extracted from microscopic slides and parasite cultures, collected in the years 2006 and 2009, we identified for the first time L. tropica as the causative agent of CL in this region. Species identification was achieved b...

The bright pigments of cave pictures in Spain have been preserved from Paeleolithic times by the accidental formation of a surface film of calcite (Kuhn, 1952), and since then many glazes have been used deliberately for the protection of colours. The idea of varnishing glass slides is not novel, but practical difficulties concerning preparation, pH, and especially viscosity, have discouraged it...