THE first description of the inclusion bodies of trachoma was made by Halberstaedter and von Prowazek following the examination of Giemsa-stained scrapings from the conjunctivae of baboons which had been inoculated with secretions from trachomatous patients (cf. Julianelle, 1938). Since then Giemsa and its modifications have held prime place as a means of demonstrating these structures and this...

Fixed chromosome preparations subjected to treatments such as pH 9 (I) or trypsin hydrolysis (2) and stained with Giemsa's demonstrate similar banding patterns irrespective of the pretreatments used. It has been shown that this phenomenon is the result of modifications of DNA-protein and protein-protein associations which alter dye interaction (3-5). It appears that only Giemsa's or related sta...

A method is described in which the relative number of adherent cells in multi-well tissue-culture plates is assayed by staining the cells with Giemsa and capturing the image of the stained cells with a video camera and charged-coupled device. The resultant image is quantified using the associated video imaging software. The method is shown to be sensitive and reproducible and should be useful f...

Fixed chromosome preparations subjected to treatments such as pH 9 (I) or trypsin hydrolysis (2) and stained with Giemsa's demonstrate similar banding patterns irrespective of the pretreatments used. It has been shown that this phenomenon is the result of modifications of DNA-protein and protein-protein associations which alter dye interaction (3-5). It appears that only Giemsa's or related sta...

Improved techniques consisting of glutaraldehyde fixation, Giemsa staining, and colony counter enumeration of foci in viral infectivity assays were compared to microscope scanning and lantern slide projection methods. These techniques broadened the scope of application to potentially hazardous viruses and increased the efficiency of assaying.

Methods An ongoing prospective study conducted from June 2012October 2013 in 90 HIV seropositive patients presenting with various complaints. CSF and lymphnode aspirates were subjected to preliminary microscopic examination, c (Giemsa staining, mucicarmine staining, negative staining and Gram’s staining). Latex agglutination test was done for CSF samples. Sampls inoculated on SDA at 37C and 25C...

We report a 63-year-old woman with myeloproliferative neoplasm (MPN), who presented with inflammatory involvement of the skin. The white cell count was 31,800/μL and the platelet count was 709×10/μL. The positivity of neutrophil alkaline phosphatase was 100% and the score was 281. The bone marrow was hypercellular with enlarged megakaryocytes with mature cytoplasm and multilobulated nuclei (Pic...

purpose: to compare the value of conventional laboratory methods and polymerase chain reaction (pcr) in the diagnosis of fungal keratitis. methods: this cross sectional study was conducted at khalili hospital, shiraz, iran. samples were taken from thirty-eight patients with findings suspicious for fungal keratitis. corneal scrapings were used for gram, giemsa and koh stains, culture and pcr ana...

This study concerns a comparative analysis of the acridine orange and Giemsa staining procedures for the fish erythrocyte micronucleus assay. The goal was to optimize the assay in the context of field water monitoring. Fish (Carassius carassius) were exposed to a reference genotoxic agent, cyclophosphamide monohydrate 5 mg l(-1) for 2, 4, and 6 days before testing. Slides from each individual w...