Gene silencing can occur either at the transcriptional level or post-transcriptional level or both. Many instances of sequence-specific silencing requires small RNAs that can be divided into two major classes: microRNAs (miRNAs) and short-interfering RNAs (siRNAs). miRNAs function in post-transcriptional gene silencing by guiding mRNA degradation or translational repression. Endogenous siRNAs a...

Macrophages can change their phenotypes according to the changes in microenvironment, and thus have various functions, that is, macrophages polarization. Macrophage phenotype is associated with progression of atherosclerotic plaques. Studies shown a large accumulation p62 protein Whether affects level macrophage polarization inflammation its mechanism not clear. The levels treated ox-LDL were d...

Genes introduced into higher plant genomes can become silent (gene silencing) and/or cause silencing of homologous genes at unlinked sites (homology-dependent gene silencing or HDG silencing). Mutations of the HOMOLOGY-DEPENDENT GENE SILENCING1 (HOG1) locus relieve transcriptional gene silencing and methylation-dependent HDG silencing and result in genome-wide demethylation. The hog1 mutant pla...

Abstract Background Gene silencing using siRNA can be a new potent strategy to treat many incurable diseases at the genetic level, including cancer and viral infections. Treatments essentially requires an efficient safe method of delivering into cells while maintaining its stability. Thus, we designed novel synergistic fusion peptides, i.e., SPACE oligoarginine. Results Among peptides siRNAs, n...

The eukaryotic translation initiation factor 5A (eIF5A) is a highly conserved protein and essential in all eukaryotes. However, the specific roles of eIF5A other biological processes remain elusive. In present study, we described role eIF5A, its posttranslational modifications (PTM), biosynthetic pathway needed for PTM Entamoeba histolytica , protozoan parasite responsible amoebic dysentery liv...

Numerous reports describe phenomena of transgene silencing in plants, yet the underlying genetic and molecular mechanisms are poorly understood. We observed that regeneration of Arabidopsis thaliana plants transgenic for the rolB gene of Agrobacterium rhizogenes results in a selection for transgene silencing. Transgene silencing could be monitored in this system by reversion of the visible RolB...