Rheumatoid arthritis (RA) is a chronic inflammatory disease that leads to the irreversible destruction of cartilage and bone. An important step in the destruction of tissue is the degradation of extracellular matrix (ECM), whose major components are type II collagen and aggrecan in cartilage, and type I collagen in bone. The synovial fluid of RA patients often contains high concentrations of pr...

Gelatinolytic activity of whole mouse spermatozoa was demonstrated by the gelatin film test. The presence of a trypsin-like protease (acrosin) in acidic extracts of mouse spermatozoa was shown by an electrophoretic method to separate the enzyme from a putative inhibitor.

Protease executes a large variety of functions and it has many biotechnological applications. It represents as one of the third largest group of industrial enzyme and finds its applications in detergents, leather industry, pharmaceutical industry etc. Among the different vegetable waste products, peel of Manihot esculenta (Tapioca/ cassava) was used as substrates for the production of protease ...

Defining the chronic cardiotoxic effects of drugs during preclinical screening is hindered by the relatively short lifetime of functional cardiac tissues in vitro, which are traditionally cultured on synthetic materials that do not recapitulate the cardiac microenvironment. Because collagen is the primary extracellular matrix protein in the heart, we hypothesized that micromolded gelatin hydrog...

The objectives of this research were to improve the film-forming properties of Channel Catfish Ictalurus punctatus skin gelatin (CSG) by cross-linking with transglutaminase (TG), determine and optimize the TG reaction time, and characterize the mechanical and barrier properties of CSG edible film. Cross-linking of CSG was performed by TG for 0, 10, 20, 30, and 40 min at 50°C, and the reaction w...

Gelatin cross-linking has recently been discovered to be a very appealing method of producing gelatin-based films. The current research looks at various commercial gelatin (type B) films improve their physical qualities. Bunium alpinum and bunium incrassatum essential oils (EOs) in two quantities (5% 25%) were added the films, which showed substantial biological activity (antibacterial, antioxi...

Film-forming solutions based on four types of biopolymers were prepared and their rheological properties determined. High methylated apple pectin sodium alginate used at the concentrations 1%, 1.5%, 2%, whereas soy protein isolate pork gelatin obtained 4%, 8%, 12%. The parameters determining production appropriate type packaging film or edible coating are setting time substrate, regardless its ...

Abstract Silk fibroin (SF) has been broadly applied in wound dressing fabrication because of its proper features for healing. In this work, we developed a carboxymethyl cellulose (CMC)/gelatin blend film with different concentrations glycerol, and modified the optimized an SF layer through electrospinning process. Tensile strength cell viability evaluation films demonstrated that glycerol conte...

There is a major focus on natural biopolymers of bacterial, animal, or plant origin as ecological materials, replacing petrochemical products. Biologically derived polylactide (PLA), polyhydroxybutyrate (PHB), and polyhydroxyalkanoates (PHA) possess interesting properties, but they are currently too expensive for most applications. Therefore, researchers try to find other that both durable chea...

Three dimensional terahertz pulsed imaging (TPI) was evaluated as a novel tool for the nondestructive characterization of different solid oral dosage forms. The time-domain reflection signal of coherent pulsed light in the far infrared was used to investigate film-coated tablets, sugar-coated tablets, multilayered controlled release tablets, and soft gelatin capsules. It is possible to determin...