This study compares the performance of a 3-h dipstick trehalose test with GLABRATA RTT, a new commercially available 20-min test for the rapid identification of Candida glabrata. With the exception of blood agar, GLABRATA RTT gave reliable results with all media tested and was always superior to the dipstick test.

STUDY DESIGN Prospective experimental study. PURPOSE To evaluate bacterial contamination during surgery. OVERVIEW OF LITERATURE The participants of surgery and ventilation system have been known as the most significant sources of contamination. METHODS Two pairs of air culture blood agar plate for G(+) bacteria and MacConkey agar plate for G(-) bacteria were placed at 3 different location...

An organism obtained from the pneumonic lungs of eleven white rats is described. It has been maintained in pure culture in a number of instances. In young cultures the organism appears as a long slender bacillus. In older cultures on coagulated serum media characteristic club-like capsular material is formed. On blood agar characteristic swellings appear at one or both ends of the rod. These be...

The use of a novobiocin-containing medium provided little benefit over observable quantitative growth on blood agar in detecting Staphylococcus saprophyticus in urine cultures.

The development and evaluation of a new selective medium (colistin-oxolinic acid-blood agar) for streptococci is described. Streptococci of medical and veterinary importance grew well on the medium. Gram-negative organisms, staphylococci. Bacillus spp., and coryneforms are all inhibited. It was concluded that the medium is valuable for the isolation of streptococci in pure culture from mixed fl...

A selective identification medium for Neisseria gonorrhoeae was devised by incorporating antibiotics and a mixture of sugars into a yeast extract agar. The results were compared with those using a standard combined selective/non-selective blood agar medium. Recovery rates of N gonorrhoeae from specimens from 208 patients cultured on both media were comparable, but the selective descriptive medi...

A previous paper (Eaton, 1934) described variants of the pneumococcus characterized by a tendency to undergo rapid spontaneous lysis under certain cultural conditions at 370C. These variants were produced by daughter-colony dissociation of smooth pneumococcus strains on blood agar at 250C. A further investigation of this method of dissociation revealed that a second and independent type of vari...

The carbohydrates of whole cells of group A, B, C, D, F, and G streptococci were analyzed with a highly sensitive gas chromatographic procedure. Characteristic chromatographic fingerprints were obtained for each group of streptococci grown in broth cultures or as single colonies on a blood agar plate. Rhamnose, glucose, and N-acetylglucosamine were major components of all the groups. Groups A, ...

No statistical differences were found in the recovery of group A streptococci from throat culture specimens after overnight incubation of blood agar plates in 5% CO2 compared with anaerobiosis. Anaerobic incubation required many more subcultures and resulted in considerably greater technical time and expense.