In chronic hypochromic anaemia the mean pepsin concentration of the gastric juice is reduced and in some patients the enzyme is totally absent (Davies, 1931; Castle, Heath, and Strauss, 1931; Meulengracht, 1932; Hartfall and Witts, 1933; Griffiths, 1934; Mullins and Flood, 1935; Aitken, Spray, and Walters, 1954). Aitken et al. (1954) divide their patients into those with and without achlorhydri...

Human gastric juice shows three separable pepsin fractions on chromatography on DEAE-cellulose. The response of these fractions to stimulation with beta-aminoethylpyrazole hydrochloride (Histalog) was studied. After a single intramuscular injection of Histalog (1.7 mg/kg body weight) there was an increase in the output of all three chromatographically distinguishable pepsin fractions. Little ch...

We have additional fi ndings regarding our study in CHEST (May 2013) 1 of gastric-to-pulmonary aspiration in adult surgical patients undergoing elective intubation. Th e original study used an enzymatic assay for identifi cation of pepsin A. Since publication, our group has validated an enzyme-linked immunosorbant assay (ELISA) for pepsin A, which we subsequently used to analyze the same airway...

Introduction: Garlic (Allium Satiyum) is a plant widely used in traditional medicine. Its anti-hypertensive, lipid lowering, oxidative activities, anti-viral, anti-bacterial, anti-fungal properties have been proven. It has a tonic effects on stomach but its effects on gastric secretion is not known. Propose of study: To investigate the effect of garlic extract on basal and pentagastrin stimulat...

Helicobacter pylori has been shown, along with other Helicobacter species to produce effector molecules that induce substantial physiological changes on acid and pepsin secretion. The effects are clinically evident, and long-term achlorhydria may be a risk factor for gastric cancer. Further identification and characterization of the factors may lead to additional understanding of gastric physio...

1. The proportion of iron liberated from twenty foods was measured after incubation in vitro with human gastric juice. 2. Results with samples of gastric juice obtained from one subject on ten occasions and from sixteen subjects on a single occasion showed good agreement, the proportions of Fe liberated covering the entire range from zero for egg to 1.0 for wine. 3. Where there was small variat...

Consumption of kiwifruit is reported to relieve symptoms of functional gastrointestinal (GI) disorder. The effect may be related to the proteases in kiwifruit. This in vitro study aimed to measure protein hydrolysis due to kiwifruit protease under gastric and duodenal conditions. A sequence of experiments incubated meat protein, with and without kiwifruit, with varying concentrations of pepsin ...

BACKGROUND Extra-esophageal carcinogenesis has been widely discussed in relation to the chronic effects of laryngopharyngeal reflux and most prominently with pepsin historically central to this discussion. With refluxate known to include gastric (pepsin) and duodenal (bile) fluids, we recently demonstrated the mechanistic role of NF-κB in mediating the preneoplastic effects of acidic-bile. Howe...

A novel substrate, albumin complexed with bromphenol blue, has been developed for the assay of human gastric juice pepsin by a kinetic method in the Cobas centrifugal analyzer. The action of pepsin on the complex degrades the albumin and releases the dye. The change in the color of the substrate is a zero-order reaction. Human and porcine pepsin have different Km's with the new substrate. This ...

Concentrations of free and total hydrogen ions, total protein and pepsin were measured in gastric juice fractions collected during basal secretion and upon stimulation by graded doses of pentagastrin administered intravenously. Undissociated hydrogen ion and non-pepsin protein concentrations were calculated as derived quantities. The studies were carried out in nine patients with duodenal ulcer...