ing from the total binding. To determine the degree of ligand depletion, aliquots were withdrawn from the supernatant in each well and counted by liquid scintillation. The experimental set-up is depicted in Figure 1. hHBP binds 3H-LPS in a saturable manner (Figure 2). By using scientific software, curvefits to the data were performed. The apparent Kd was determined to be 90 ± 5 ng/mL and the Bm...

With respect to paragraph 6 of Dr. L0vborg's letter, the solid phase was blocked with BSA and Tween as described. The assessment of the plates did not occur in only a few experiments. We worked with the Nunc plates using the complete ELISA procedure as described for many months before evaluating other types of plates. Finally, the view held by Dr. L0vborg in paragraph 3 that detergent is a weak...

Different tests for the identification of Gardnerella (Haemophilus) vaginalis and for its differentiation from catalase-negative unclassified coryneforms from the vagina were evaluated on over 200 bacterial strains, with special emphasis on optimal test conditions. A presumptive identification of G. vaginalis in the clinical laboratory can be made on the basis of colonial morphology, clear beta...

Background and objectives: Persian scholars such as Avicenna and Zakariya al-Razi have pointed out many uses for henna oil. The present study aimed to propose a method for standardization of this oil. Methods: The luteolin content has been evaluated quantitively by HPTLC method to standardize the henna oil. The oil sample was applied on silicagel plate and the ...

Aims. The treatment modalities of patients with vaginal discharge are generally related to their symptoms. The aim of this study was to evaluate vaginal flora and antibiogram analysis in reproductive-age women with or without vaginitis in primary care settings. Methods. Vaginal swabs were taken from 311 women who have vaginitis, and tested for the causative agents of vaginal discharge. The cont...

Vaginal swabs were examined for the presence of Gardnerella vaginalis. Of 294 isolates with appropriate colonial and cellular morphology subjected to an identification procedure, 203 (69%) were identified as G vaginalis. The 91 isolates not identified as G vaginalis were differentiated by their inability to ferment starch, cause diffuse beta haemolysis on human blood agar or hydrolyse hippurate...

Bacterial vaginosis is a highly prevalent and poorly understood polymicrobial disorder of the vaginal microbiota, with significant adverse sequelae. Gardnerella vaginalis predominates in bacterial vaginosis. Biofilms of G. vaginalis are present in human infections and are implicated in persistent disease, treatment failure, and transmission. Here we demonstrate that G. vaginalis biofilms contai...

A predominance of Lactobacillus species in the vaginal flora is considered normal. In women with bacterial vaginosis, the prevalence and concentrations of intravaginal Gardnerella vaginalis and anaerobes are increased, whereas the prevalence of intravaginal Lactobacillus species is decreased. Because some lactobacilli are known to produce hydrogen peroxide (H2O2), which can be toxic to organism...

BACKGROUND We aimed to determine the prevalence of vaginal colonization by Gardnerella vaginalis and of bacterial vaginosis (BV) in Portuguese pregnant women, and to identify risk factors for BV and G. vaginalis colonization in pregnancy. METHODS A cross-sectional study was conducted among pregnant women aged ≥ 18 years who were attending in two public hospitals of the Northwest region of Por...